BMRB: 10163

Solution structure of the 24th filamin domain from human Filamin-B

Authors

Tomizawa, T., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

Filamin-B

Entity

1. Filamin-B (polymer, Thiol state: all free), 101 monomers, 10526.71 Da Detail

GSSGSSGSSD ASKVTSKGAG LSKAFVGQKS SFLVDCSKAG SNMLLIGVHG PTTPCEEVSM KHVGNQQYNV TYVVKERGDY VLAVKWGEEH IPGSPFHVTV P

Formula weight

10526.71 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.0 %, Completeness: 96.3 %, Completeness (bb): 93.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.3 % (1055 of 1096)	96.6 % (544 of 563)	96.5 % (415 of 430)	93.2 % (96 of 103)
Backbone	93.5 % (557 of 596)	92.9 % (195 of 210)	94.1 % (273 of 290)	92.7 % (89 of 96)
Sidechain	97.4 % (573 of 588)	97.2 % (343 of 353)	97.8 % (223 of 228)	100.0 % (7 of 7)
Aromatic	100.0 % (82 of 82)	100.0 % (41 of 41)	100.0 % (40 of 40)	100.0 % (1 of 1)
Methyl	100.0 % (100 of 100)	100.0 % (50 of 50)	100.0 % (50 of 50)

1. filamin domain

GSSGSSGSSD ASKVTSKGAG LSKAFVGQKS SFLVDCSKAG SNMLLIGVHG PTTPCEEVSM KHVGNQQYNV TYVVKERGDY VLAVKWGEEH IPGSPFHVTV P

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	filamin domain	[U-13C; U-15N]	protein	1.11 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.0 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.0 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.16 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.61 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EED, Strand ID: A Detail

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Release date

2009-02-19

Citation

Solution structure of the 24th filamin domain from human Filamin-B
Tomizawa, T., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Related entities 1. Filamin-B, : 1 : 4 : 495 entities Detail

Interaction partners 1. Filamin-B, : 68 interactors Detail

Experiments performed 2 experiments Detail