BMRB: 10164

Solution structure of the A1pp domain from human protein C6orf130

Authors

Tomizawa, T., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

Uncharacterized protein C6orf130

Entity

1. Uncharacterized protein C6orf130 (polymer, Thiol state: all free), 149 monomers, 16469.86 Da Detail

GSSGSSGGSR ITYVKGDLFA CPKTDSLAHC ISEDCRMGAG IAVLFKKKFG GVQELLNQQK KSGEVAVLKR DGRYIYYLIT KKRASHKPTY ENLQKSLEAM KSHCLKNGVT DLSMPRIGCG LDRLQWENVS AMIEEVFEAT DIKITVYTL

Formula weight

16469.86 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.3 %, Completeness: 95.1 %, Completeness (bb): 93.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.1 % (1640 of 1725)	95.1 % (855 of 899)	95.8 % (642 of 670)	91.7 % (143 of 156)
Backbone	93.2 % (828 of 888)	92.2 % (285 of 309)	94.7 % (410 of 433)	91.1 % (133 of 146)
Sidechain	96.7 % (940 of 972)	96.6 % (570 of 590)	96.8 % (360 of 372)	100.0 % (10 of 10)
Aromatic	100.0 % (112 of 112)	100.0 % (56 of 56)	100.0 % (55 of 55)	100.0 % (1 of 1)
Methyl	99.4 % (161 of 162)	100.0 % (81 of 81)	98.8 % (80 of 81)

1. A1pp domain

GSSGSSGGSR ITYVKGDLFA CPKTDSLAHC ISEDCRMGAG IAVLFKKKFG GVQELLNQQK KSGEVAVLKR DGRYIYYLIT KKRASHKPTY ENLQKSLEAM KSHCLKNGVT DLSMPRIGCG LDRLQWENVS AMIEEVFEAT DIKITVYTL

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	A1pp domain	[U-13C; U-15N]	protein	1.22 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.05 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.05 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.1 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EEE, Strand ID: A Detail

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Release date

2009-02-22

Citation

Solution structure of the A1pp domain from human protein C6orf130
Tomizawa, T., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Related entities 1. Uncharacterized protein C6orf130, : 1 : 7 : 22 entities Detail

Interaction partners 1. Uncharacterized protein C6orf130, : 2 interactors Detail

Experiments performed 2 experiments Detail