BMRB: 10166

Solution structure of the C2H2 type zinc finger (region 637-667) of human Zinc finger protein 268

Authors

Tomizawa, T., Tochio, N., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.

Assembly

Zinc finger protein 268

Entity

1. zf-C2H2 domain (polymer, Thiol state: all other bound), 44 monomers, 4425.867 Da Detail

GSSGSSGEKP YSCNECGKAF TFKSQLIVHK GVHTGVKPSG PSSG

2. ZN (non-polymer), na Da

Formula weight

4425.867 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS13:SG	2:ZN1:ZN
2	na	sing	1:CYS16:SG	2:ZN1:ZN
3	na	sing	1:HIS29:NE2	2:ZN1:ZN
4	na	sing	1:HIS33:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 81.8 %, Completeness: 85.2 %, Completeness (bb): 78.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	85.2 % (397 of 466)	85.6 % (208 of 243)	86.7 % (156 of 180)	76.7 % (33 of 43)
Backbone	78.3 % (202 of 258)	76.3 % (71 of 93)	80.6 % (100 of 124)	75.6 % (31 of 41)
Sidechain	92.2 % (225 of 244)	91.3 % (137 of 150)	93.5 % (86 of 92)	100.0 % (2 of 2)
Aromatic	100.0 % (36 of 36)	100.0 % (18 of 18)	100.0 % (18 of 18)
Methyl	100.0 % (26 of 26)	100.0 % (13 of 13)	100.0 % (13 of 13)

1. zf-C2H2 domain

GSSGSSGEKP YSCNECGKAF TFKSQLIVHK GVHTGVKPSG PSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Zinc finger protein 268	[U-13C; U-15N]	protein	1.0 (±0.1) mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	ZnCl2		salt	0.05 mM
5	IDA		salt	1 mM
6	d-DTT		salt	1 mM
7	NaN3		salt	0.02 %
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EM1, Strand ID: A Detail

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Release date

2009-02-22

Citation

Solution structure of the C2H2 type zinc finger (region 637-667) of human Zinc finger protein 268
Tochio, N., Tomizawa, T., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.

Related entities 1. zf-C2H2 domain, : 1 : 266 entities Detail

Experiments performed 2 experiments Detail