Solution structure of the C2H2 type zinc finger (region 584-616) of human Zinc finger protein 28 homolog
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | na | sing | 1:CYS15:SG | 2:ZN1:ZN |
2 | na | sing | 1:CYS18:SG | 2:ZN1:ZN |
3 | na | sing | 1:HIS31:NE2 | 2:ZN1:ZN |
4 | na | sing | 1:HIS35:NE2 | 2:ZN1:ZN |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 77.6 % (385 of 496) | 78.3 % (206 of 263) | 79.3 % (149 of 188) | 66.7 % (30 of 45) |
Backbone | 69.3 % (187 of 270) | 67.0 % (65 of 97) | 72.3 % (94 of 130) | 65.1 % (28 of 43) |
Sidechain | 86.0 % (227 of 264) | 84.9 % (141 of 166) | 87.5 % (84 of 96) | 100.0 % (2 of 2) |
Aromatic | 100.0 % (32 of 32) | 100.0 % (16 of 16) | 100.0 % (16 of 16) | |
Methyl | 100.0 % (22 of 22) | 100.0 % (11 of 11) | 100.0 % (11 of 11) |
1. zf-C2H2
GSSGSSGSGE KPFKCKECGK AFRQNIHLAS HLRIHTGEKP SGPSSGSolvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Zinc finger protein 28 homolog | [U-13C; U-15N] | protein | 1.0 mM |
2 | d-Tris-HCl | buffer | 20 mM | |
3 | NaCl | salt | 100 mM | |
4 | ZnCl2 | salt | 0.05 mM | |
5 | IDA | salt | 1 mM | |
6 | d-DTT | salt | 1 mM | |
7 | NaN3 | salt | 0.02 % | |
8 | H2O | solvent | 90 % | |
9 | D2O | solvent | 10 % |