Solution structure of the C2H2 type zinc finger (region 311-343) of human Zinc finger protein 224
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | na | sing | 1:CYS15:SG | 2:ZN1:ZN |
2 | na | sing | 1:CYS18:SG | 2:ZN1:ZN |
3 | na | sing | 1:HIS31:NE2 | 2:ZN1:ZN |
4 | na | sing | 1:HIS35:NE2 | 2:ZN1:ZN |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 81.8 % (396 of 484) | 81.3 % (208 of 256) | 84.2 % (154 of 183) | 75.6 % (34 of 45) |
Backbone | 76.3 % (206 of 270) | 72.6 % (69 of 95) | 79.5 % (105 of 132) | 74.4 % (32 of 43) |
Sidechain | 87.8 % (223 of 254) | 86.3 % (139 of 161) | 90.1 % (82 of 91) | 100.0 % (2 of 2) |
Aromatic | 100.0 % (28 of 28) | 100.0 % (14 of 14) | 100.0 % (14 of 14) | |
Methyl | 100.0 % (18 of 18) | 100.0 % (9 of 9) | 100.0 % (9 of 9) |
1. zf-C2H2
GSSGSSGTAE KPFRCDTCDK SFRQRSALNS HRMIHTGEKP SGPSSGSolvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | zf-C2H2 | [U-13C; U-15N] | protein | 1.0 (±0.1) mM |
2 | d-Tris-HCl | buffer | 20 mM | |
3 | NaCl | salt | 100 mM | |
4 | ZnCl2 | salt | 0.05 mM | |
5 | IDA | salt | 1 mM | |
6 | d-DTT | salt | 1 mM | |
7 | NaN3 | salt | 0.02 % | |
8 | H2O | solvent | 90 % | |
9 | D2O | solvent | 10 % |