BMRB: 10172

Solution structure of the C2H2 type zinc finger (region 395-427) of human Zinc finger protein 224

Authors

Tochio, N., Tomizawa, T., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.

Assembly

Zinc finger protein 224

Entity

1. zf-C2H2 domain (polymer, Thiol state: free and other bound), 46 monomers, 4728.950 Da Detail

GSSGSSGSGE KPFKCEECGK GFYTNSQCYS HQRSHSGEKP SGPSSG

2. ZN (non-polymer), 65.409 Da

Total weight

4794.3594 Da

Max. entity weight

4728.95 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS15:SG	2:ZN1:ZN
2	na	sing	1:CYS18:SG	2:ZN1:ZN
3	na	sing	1:HIS31:NE2	2:ZN1:ZN
4	na	sing	1:HIS35:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 76.1 %, Completeness: 80.1 %, Completeness (bb): 74.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	80.1 % (382 of 477)	80.6 % (204 of 253)	80.9 % (144 of 178)	73.9 % (34 of 46)
Backbone	74.4 % (201 of 270)	74.5 % (73 of 98)	75.2 % (97 of 129)	72.1 % (31 of 43)
Sidechain	85.7 % (209 of 244)	84.5 % (131 of 155)	87.2 % (75 of 86)	100.0 % (3 of 3)
Aromatic	100.0 % (44 of 44)	100.0 % (22 of 22)	100.0 % (22 of 22)
Methyl	100.0 % (2 of 2)	100.0 % (1 of 1)	100.0 % (1 of 1)

1. zf-C2H2 domain

GSSGSSGSGE KPFKCEECGK GFYTNSQCYS HQRSHSGEKP SGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	zf-C2H2 domain	[U-13C; U-15N]	protein	1.0 (±0.1) mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	ZnCl2		salt	0.05 mM
5	IDA		salt	1 mM
6	d-DTT		salt	1 mM
7	NaN3		salt	0.02 %
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2ENC, Strand ID: A Detail

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Release date

2009-02-23

Citation

Solution structure of the C2H2 type zinc finger (region 395-427) of human Zinc finger protein 224
Tochio, N., Tomizawa, T., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.

Related entities 1. zf-C2H2 domain, : 1 : 194 entities Detail

Experiments performed 2 experiments Detail