BMRB: 10176

Solution structure of the C2H2 type zinc finger (region 696-728) of human Zinc finger protein 28 homolog

Authors

Tochio, N., Tomizawa, T., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.

Assembly

Zinc finger protein 28 homolog

Entity

1. zf-C2H2 (polymer, Thiol state: all other bound), 46 monomers, 4688.011 Da Detail

GSSGSSGTGE KPYKCMECGK AFGDNSSCTQ HQRLHTGQRP SGPSSG

2. ZN (non-polymer), 65.409 Da

Total weight

4753.4204 Da

Max. entity weight

4688.011 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS15:SG	2:ZN1:ZN
2	na	sing	1:CYS18:SG	2:ZN1:ZN
3	na	sing	1:HIS31:NE2	2:ZN1:ZN
4	na	sing	1:HIS35:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 78.3 %, Completeness: 80.8 %, Completeness (bb): 75.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	80.8 % (379 of 469)	81.0 % (201 of 248)	82.8 % (144 of 174)	72.3 % (34 of 47)
Backbone	75.9 % (205 of 270)	74.5 % (73 of 98)	78.3 % (101 of 129)	72.1 % (31 of 43)
Sidechain	86.4 % (204 of 236)	85.3 % (128 of 150)	89.0 % (73 of 82)	75.0 % (3 of 4)
Aromatic	100.0 % (26 of 26)	100.0 % (13 of 13)	100.0 % (13 of 13)
Methyl	100.0 % (12 of 12)	100.0 % (6 of 6)	100.0 % (6 of 6)

1. zf-C2H2

GSSGSSGTGE KPYKCMECGK AFGDNSSCTQ HQRLHTGQRP SGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	zf-C2H2	[U-13C; U-15N]	protein	1.0 (±0.1) mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	ZnCl2		salt	0.05 mM
5	IDA		salt	1 mM
6	d-DTT		salt	1 mM
7	NaN3		salt	0.02 %
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2YTM, Strand ID: A Detail

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Release date

2009-02-23

Citation

Solution structure of the C2H2 type zinc finger (region 696-728) of human Zinc finger protein 28 homolog
Tochio, N., Tomizawa, T., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.

Related entities 1. zf-C2H2, : 1 : 135 entities Detail

Experiments performed 2 experiments Detail