BMRB: 10178

Solution structure of the C2H2 type zinc finger (region 204-236) of human Zinc finger protein 473

Authors

Tochio, N., Tomizawa, T., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.

Assembly

Zinc finger protein 473

Entity

1. zf-C2H2 (polymer, Thiol state: all other bound), 46 monomers, 4863.148 Da Detail

GSSGSSGEGE KPYQCSECGK SFSGSYRLTQ HWITHTREKP SGPSSG

2. ZN (non-polymer), 65.409 Da

Total weight

4928.557 Da

Max. entity weight

4863.148 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS15:SG	2:ZN1:ZN
2	na	sing	1:CYS18:SG	2:ZN1:ZN
3	na	sing	1:HIS31:NE2	2:ZN1:ZN
4	na	sing	1:HIS35:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 76.1 %, Completeness: 76.7 %, Completeness (bb): 72.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	76.7 % (376 of 490)	77.1 % (199 of 258)	78.0 % (145 of 186)	69.6 % (32 of 46)
Backbone	72.2 % (195 of 270)	71.1 % (69 of 97)	73.8 % (96 of 130)	69.8 % (30 of 43)
Sidechain	81.8 % (211 of 258)	80.7 % (130 of 161)	84.0 % (79 of 94)	66.7 % (2 of 3)
Aromatic	73.9 % (34 of 46)	73.9 % (17 of 23)	77.3 % (17 of 22)	0.0 % (0 of 1)
Methyl	100.0 % (14 of 14)	100.0 % (7 of 7)	100.0 % (7 of 7)

1. zf-C2H2

GSSGSSGEGE KPYQCSECGK SFSGSYRLTQ HWITHTREKP SGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	sample	[U-13C; U-15N]	protein	1.0 (±0.1) mM
2	d-Tris-HCl		buffer	20 mM
3	ZnCl2		salt	0.05 mM
4	IDA		salt	1 mM
5	d-DTT		salt	1 mM
6	NaN3		salt	0.02 %
7	H2O		solvent	90 %
8	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.05 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2YTT, Strand ID: A Detail

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Release date

2009-02-23

Citation

Solution structure of the C2H2 type zinc finger (region 204-236) of human Zinc finger protein 473
Tochio, N., Tomizawa, T., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.

Related entities 1. zf-C2H2, : 1 : 119 entities Detail

Experiments performed 2 experiments Detail