Solution structure of the C2H2 type zinc finger (region 724-756) of human Zinc finger protein 28 homolog
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | na | sing | 1:CYS15:SG | 2:ZN1:ZN |
2 | na | sing | 1:CYS18:SG | 2:ZN1:ZN |
3 | na | sing | 1:HIS31:NE2 | 2:ZN1:ZN |
4 | na | sing | 1:HIS35:NE2 | 2:ZN1:ZN |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 81.1 % (391 of 482) | 82.4 % (210 of 255) | 82.5 % (151 of 183) | 68.2 % (30 of 44) |
Backbone | 72.6 % (196 of 270) | 70.1 % (68 of 97) | 76.2 % (99 of 130) | 67.4 % (29 of 43) |
Sidechain | 90.8 % (227 of 250) | 89.9 % (142 of 158) | 92.3 % (84 of 91) | 100.0 % (1 of 1) |
Aromatic | 100.0 % (26 of 26) | 100.0 % (13 of 13) | 100.0 % (13 of 13) | |
Methyl | 95.0 % (19 of 20) | 100.0 % (10 of 10) | 90.0 % (9 of 10) |
1. zf-C2H2 domain
GSSGSSGTGQ RPYECIECGK AFKTKSSLIC HRRSHTGEKP SGPSSGSolvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | zf-C2H2 domain | [U-13C; U-15N] | protein | 1.0 (±0.1) mM |
2 | d-Tris-HCl | buffer | 20 mM | |
3 | NaCl | salt | 100 mM | |
4 | ZnCl2 | salt | 0.05 mM | |
5 | IDA | salt | 1 mM | |
6 | d-DTT | salt | 1 mM | |
7 | NaN3 | salt | 0.02 % | |
8 | H2O | solvent | 90 % | |
9 | D2O | solvent | 10 % |