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Solution structure of the C2H2 type zinc finger (region 725-757) of human Zinc finger protein 473
Authors
Tomizawa, T., Tochio, N., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.
Assembly
Zinc finger protein 473
Entity
1. zf-C2H2 domain (polymer, Thiol state: all other bound), 46 monomers, 4669.047 Da Detail

GSSGSSGSGE KPYVCDYCGK AFGLSAELVR HQRIHTGEKP SGPSSG


2. ZN (non-polymer), 65.409 Da
Total weight
4734.456 Da
Max. entity weight
4669.047 Da
Entity Connection
na 4 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1nasing1:CYS15:SG2:ZN1:ZN
2nasing1:CYS18:SG2:ZN1:ZN
3nasing1:HIS31:NE22:ZN1:ZN
4nasing1:HIS35:NE22:ZN1:ZN

Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
torsion angle dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 80.4 %, Completeness: 77.9 %, Completeness (bb): 70.4 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All77.9 % (370 of 475)78.6 % (195 of 248)78.1 % (143 of 183)72.7 % (32 of 44)
Backbone70.4 % (190 of 270)68.4 % (67 of 98)71.3 % (92 of 129)72.1 % (31 of 43)
Sidechain86.8 % (210 of 242)85.3 % (128 of 150)89.0 % (81 of 91)100.0 % (1 of 1)
Aromatic100.0 % (34 of 34)100.0 % (17 of 17)100.0 % (17 of 17)
Methyl88.5 % (23 of 26)92.3 % (12 of 13)84.6 % (11 of 13)

1. zf-C2H2 domain

GSSGSSGSGE KPYVCDYCGK AFGLSAELVR HQRIHTGEKP SGPSSG

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)


#NameIsotope labelingTypeConcentration
1zf-C2H2 domain[U-13C; U-15N]protein1.0 (±0.1) mM
2d-Tris-HClbuffer20 mM
3NaClsalt100 mM
4ZnCl2salt0.05 mM
5IDAsalt1 mM
6d-DTTsalt1 mM
7NaN3salt0.02 %
8H2Osolvent90 %
9D2Osolvent10 %

LACS Plot; CA
Referencing offset: -0.11 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.11 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: 0.04 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: -0.36 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2EME, Strand ID: A Detail


Release date
2009-02-25
Citation
Solution structure of the C2H2 type zinc finger (region 725-757) of human Zinc finger protein 473
Tochio, N., Tomizawa, T., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.
Related entities 1. zf-C2H2 domain, : 1 : 194 entities Detail
Experiments performed 2 experiments Detail
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