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Solution structure of the C2H2 type zinc finger (region 341-373) of human Zinc finger protein 95 homolog
Authors
Tochio, N., Tomizawa, T., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.
Assembly
Zinc finger protein 95 homolog
Entity
1. zf-C2H2 (polymer, Thiol state: all other bound), 46 monomers, 4842.188 Da Detail

GSSGSSGHGE RGHRCSDCGK FFLQASNFIQ HRRIHTGEKP SGPSSG


2. ZN (non-polymer), 65.409 Da
Total weight
4907.597 Da
Max. entity weight
4842.188 Da
Entity Connection
na 4 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1nasing1:CYS15:SG2:ZN1:ZN
2nasing1:CYS18:SG2:ZN1:ZN
3nasing1:HIS31:NE22:ZN1:ZN
4nasing1:HIS35:NE22:ZN1:ZN

Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
torsion angle dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 87.0 %, Completeness: 87.3 %, Completeness (bb): 79.0 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All87.3 % (427 of 489)87.6 % (226 of 258)90.2 % (166 of 184)74.5 % (35 of 47)
Backbone79.0 % (215 of 272)75.8 % (75 of 99)83.7 % (108 of 129)72.7 % (32 of 44)
Sidechain92.5 % (235 of 254)91.2 % (145 of 159)94.6 % (87 of 92)100.0 % (3 of 3)
Aromatic100.0 % (46 of 46)100.0 % (23 of 23)100.0 % (23 of 23)
Methyl100.0 % (16 of 16)100.0 % (8 of 8)100.0 % (8 of 8)

1. zf-C2H2

GSSGSSGHGE RGHRCSDCGK FFLQASNFIQ HRRIHTGEKP SGPSSG

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)


#NameIsotope labelingTypeConcentration
1zf-C2H2[U-13C; U-15N]protein1.0 mM
2d-Tris-HClbuffer20 mM
3NaClsalt100 mM
4ZnCl2salt0.05 mM
5IDAsalt1 mM
6d-DTTsalt1 mM
7NaN3salt0.02 %
8H2Osolvent90 %
9D2Osolvent10 %

LACS Plot; CA
Referencing offset: -0.18 ppm, Outliers: 3 Detail
LACS Plot; CB
Referencing offset: -0.18 ppm, Outliers: 3 Detail
LACS Plot; HA
Referencing offset: 0.03 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: -0.12 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2EOM, Strand ID: A Detail


Release date
2009-02-26
Citation
Solution structure of the C2H2 type zinc finger (region 341-373) of human Zinc finger protein 95 homolog
Tochio, N., Tomizawa, T., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.
Related entities 1. zf-C2H2, : 1 : 128 entities Detail
Experiments performed 2 experiments Detail
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