Solution structure of the C2H2 type zinc finger (region 719-751) of human Zinc finger protein 268
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | na | sing | 1:CYS15:SG | 2:ZN1:ZN |
2 | na | sing | 1:CYS18:SG | 2:ZN1:ZN |
3 | na | sing | 1:HIS31:NE2 | 2:ZN1:ZN |
4 | na | sing | 1:HIS35:NE2 | 2:ZN1:ZN |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 79.4 % (382 of 481) | 77.9 % (197 of 253) | 82.9 % (150 of 181) | 74.5 % (35 of 47) |
Backbone | 75.6 % (204 of 270) | 71.1 % (69 of 97) | 80.0 % (104 of 130) | 72.1 % (31 of 43) |
Sidechain | 84.3 % (210 of 249) | 82.1 % (128 of 156) | 87.6 % (78 of 89) | 100.0 % (4 of 4) |
Aromatic | 100.0 % (32 of 32) | 100.0 % (16 of 16) | 100.0 % (16 of 16) | |
Methyl | 100.0 % (20 of 20) | 100.0 % (10 of 10) | 100.0 % (10 of 10) |
1. zf-C2H2, UNP residues 719-751
GSSGSSGTGE KPHECRECGK SFSFNSQLIV HQRIHTGENP SGPSSGSolvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | zf-C2H2, UNP residues 719-751 | [U-13C; U-15N] | protein | 1.0 (±0.1) mM |
2 | d-Tris-HCl | buffer | 20 mM | |
3 | NaCl | salt | 100 mM | |
4 | ZnCl2 | salt | 0.05 mM | |
5 | IDA | salt | 1 mM | |
6 | d-DTT | salt | 1 mM | |
7 | NaN3 | salt | 0.02 % | |
8 | H2O | solvent | 90 % | |
9 | D2O | solvent | 10 % |