BMRB: 10193

Solution structure of the Myb-like DNA-binding domain of human ZZZ3 protein

Authors

Abe, H., Tochio, N., Miyamoto, K., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Zinc finger ZZ-type-containing protein 3

Entity

1. Zinc finger ZZ-type-containing protein 3 (polymer, Thiol state: not present), 75 monomers, 8288.251 Da Detail

GSSGSSGNQL WTVEEQKKLE QLLIKYPPEE VESRRWQKIA DELGNRTAKQ VASQVQKYFI KLTKAGIPVS GPSSG

Formula weight

8288.251 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.0 %, Completeness: 90.9 %, Completeness (bb): 86.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.9 % (807 of 888)	91.5 % (430 of 470)	91.4 % (307 of 336)	85.4 % (70 of 82)
Backbone	86.0 % (380 of 442)	85.0 % (130 of 153)	86.7 % (189 of 218)	85.9 % (61 of 71)
Sidechain	95.3 % (490 of 514)	94.6 % (300 of 317)	97.3 % (181 of 186)	81.8 % (9 of 11)
Aromatic	100.0 % (50 of 50)	100.0 % (25 of 25)	100.0 % (23 of 23)	100.0 % (2 of 2)
Methyl	100.0 % (74 of 74)	100.0 % (37 of 37)	100.0 % (37 of 37)

1. Myb-like DNA-binding domain, UNP residues 652-713

GSSGSSGNQL WTVEEQKKLE QLLIKYPPEE VESRRWQKIA DELGNRTAKQ VASQVQKYFI KLTKAGIPVS GPSSG

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Myb-like DNA-binding domain, UNP residues 652-713	[U-13C; U-15N]	protein	1.14 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.15 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.15 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.69 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2YUM, Strand ID: A Detail

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Release date

2009-02-26

Citation

Solution structure of the Myb-like DNA-binding domain of human ZZZ3 protein
Abe, H., Tochio, N., Miyamoto, K., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Zinc finger ZZ-type-containing protein 3, : 1 : 42 entities Detail

Interaction partners 1. Zinc finger ZZ-type-containing protein 3, : 16 interactors Detail

Experiments performed 2 experiments Detail