BMRB: 10194

Solution structure of the SH3 domain of mouse RUN and TBC1 domain containing 3

Authors

Abe, H., Tochio, N., Miyamoto, K., Saito, K., Sasagawa, A., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

RUN and TBC1 domain containing 3

Entity

1. RUN and TBC1 domain containing 3 (polymer, Thiol state: all free), 78 monomers, 8669.455 Da Detail

GSSGSSGRRA KALLDFERHD DDELGFRKND IITIISQKDE HCWVGELNGL RGWFPAKFVE VLDERSKEYS IASGPSSG

Formula weight

8669.455 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.6 %, Completeness: 91.3 %, Completeness (bb): 87.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.3 % (809 of 886)	91.4 % (423 of 463)	92.1 % (315 of 342)	87.7 % (71 of 81)
Backbone	87.7 % (407 of 464)	86.5 % (141 of 163)	88.9 % (200 of 225)	86.8 % (66 of 76)
Sidechain	94.7 % (465 of 491)	94.0 % (282 of 300)	95.7 % (178 of 186)	100.0 % (5 of 5)
Aromatic	100.0 % (80 of 80)	100.0 % (40 of 40)	100.0 % (38 of 38)	100.0 % (2 of 2)
Methyl	100.0 % (66 of 66)	100.0 % (33 of 33)	100.0 % (33 of 33)

1. SH3 domain

GSSGSSGRRA KALLDFERHD DDELGFRKND IITIISQKDE HCWVGELNGL RGWFPAKFVE VLDERSKEYS IASGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain	[U-13C; U-15N]	protein	1.00 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	ZnCl2		salt	0.05 mM
7	IDA		salt	1 mM
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.13 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.13 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.4 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2YUO, Strand ID: A Detail

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Release date

2009-02-26

Citation

Solution structure of the SH3 domain of mouse RUN and TBC1 domain containing 3
Abe, H., Tochio, N., Miyamoto, K., Saito, K., Sasagawa, A., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. RUN and TBC1 domain containing 3, : 1 : 4 : 118 entities Detail

Interaction partners 1. RUN and TBC1 domain containing 3, : 1 interactors Detail

Experiments performed 2 experiments Detail