Solution structure of the second SH3 domain of human Vinexin
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 90.4 % (922 of 1020) | 91.8 % (490 of 534) | 89.3 % (352 of 394) | 87.0 % (80 of 92) |
Backbone | 87.5 % (464 of 530) | 90.4 % (169 of 187) | 86.0 % (222 of 258) | 85.9 % (73 of 85) |
Sidechain | 93.5 % (531 of 568) | 92.5 % (321 of 347) | 94.9 % (203 of 214) | 100.0 % (7 of 7) |
Aromatic | 100.0 % (86 of 86) | 100.0 % (43 of 43) | 100.0 % (42 of 42) | 100.0 % (1 of 1) |
Methyl | 97.4 % (76 of 78) | 97.4 % (38 of 39) | 97.4 % (38 of 39) |
1. SH3 domain
GSSGSSGKPP TYQVLEYGEA VAQYTFKGDL EVELSFRKGE HICLIRKVNE NWYEGRITGT GRQGIFPASY VQVSREPRLR LCDDSGPSSGSolvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | SH3 domain | [U-13C; U-15N] | protein | 1.00 mM |
2 | d-Tris-HCl | buffer | 20 mM | |
3 | NaCl | salt | 100 mM | |
4 | d-DTT | salt | 1 mM | |
5 | NaN3 | salt | 0.02 % | |
6 | H2O | solvent | 90 % | |
7 | D2O | solvent | 10 % |