BMRB: 10196

Solution structure of the C2H2 type zinc finger (region 768-800) of human Zinc finger protein 95 homolog

Authors

Tomizawa, T., Tochio, N., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.

Assembly

Zinc finger protein 95 homolog

Entity

1. zf-C2H2, UNP residues 768-800 (polymer, Thiol state: all other bound), 46 monomers, 4762.054 Da Detail

GSSGSSGSST KSHQCHECGR GFTLKSHLNQ HQRIHTGEKP SGPSSG

2. ZN (non-polymer), 65.409 Da

Total weight

4827.4634 Da

Max. entity weight

4762.054 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS15:SG	2:ZN1:ZN
2	na	sing	1:CYS18:SG	2:ZN1:ZN
3	na	sing	1:HIS31:NE2	2:ZN1:ZN
4	na	sing	1:HIS35:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 78.3 %, Completeness: 78.5 %, Completeness (bb): 71.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	78.5 % (376 of 479)	78.2 % (197 of 252)	81.0 % (145 of 179)	70.8 % (34 of 48)
Backbone	71.0 % (193 of 272)	67.3 % (66 of 98)	74.6 % (97 of 130)	68.2 % (30 of 44)
Sidechain	81.6 % (200 of 245)	80.5 % (124 of 154)	82.8 % (72 of 87)	100.0 % (4 of 4)
Aromatic	66.7 % (20 of 30)	66.7 % (10 of 15)	66.7 % (10 of 15)
Methyl	100.0 % (18 of 18)	100.0 % (9 of 9)	100.0 % (9 of 9)

1. zf-C2H2, UNP residues 768-800

GSSGSSGSST KSHQCHECGR GFTLKSHLNQ HQRIHTGEKP SGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	zf-C2H2, UNP residues 768-800	[U-13C; U-15N]	protein	1.0 (±0.1) mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	ZnCl2		salt	0.05 mM
5	IDA		salt	1 mM
6	d-DTT		salt	1 mM
7	NaN3		salt	0.02 %
8	D2O		solvent	10 %
9	H2O		solvent	90 %

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LACS Plot; CA

Referencing offset: -0.12 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.12 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.07 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.22 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EM5, Strand ID: A Detail

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Release date

2009-03-04

Citation

Solution structure of the C2H2 type zinc finger (region 768-800) of human Zinc finger protein 95 homolog
Tochio, N., Tomizawa, T., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.

Related entities 1. zf-C2H2, UNP residues 768-800, : 1 : 50 entities Detail

Experiments performed 2 experiments Detail