Solution structure of the C2H2 type zinc finger (region 423-455) of human Zinc finger protein 224
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | na | sing | 1:CYS15:SG | 2:ZN1:ZN |
2 | na | sing | 1:CYS18:SG | 2:ZN1:ZN |
3 | na | sing | 1:HIS31:NE2 | 2:ZN1:ZN |
4 | na | sing | 1:HIS35:NE2 | 2:ZN1:ZN |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 86.3 % (430 of 498) | 87.1 % (230 of 264) | 87.3 % (165 of 189) | 77.8 % (35 of 45) |
Backbone | 79.8 % (217 of 272) | 78.8 % (78 of 99) | 81.4 % (105 of 129) | 77.3 % (34 of 44) |
Sidechain | 92.8 % (244 of 263) | 92.1 % (152 of 165) | 93.8 % (91 of 97) | 100.0 % (1 of 1) |
Aromatic | 100.0 % (34 of 34) | 100.0 % (17 of 17) | 100.0 % (17 of 17) | |
Methyl | 100.0 % (22 of 22) | 100.0 % (11 of 11) | 100.0 % (11 of 11) |
1. zf-C2H2
GSSGSSGSGE KPYKCVECGK GYKRRLDLDF HQRVHTGEKL SGPSSGSolvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | zf-C2H2 | [U-13C; U-15N] | protein | 1.0 mM |
2 | d-Tris-HCl | buffer | 20 mM | |
3 | NaCl | salt | 100 mM | |
4 | ZnCl2 | salt | 0.05 mM | |
5 | IDA | salt | 1 mM | |
6 | d-DTT | salt | 1 mM | |
7 | NaN3 | salt | 0.02 % | |
8 | H2O | solvent | 90 % | |
9 | D2O | solvent | 10 % |