Solution structure of the C2H2 type zinc finger (region 342-372) of human Zinc finger protein 473
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | na | sing | 1:CYS15:SG | 2:ZN1:ZN |
2 | na | sing | 1:CYS18:SG | 2:ZN1:ZN |
3 | na | sing | 1:HIS31:NE2 | 2:ZN1:ZN |
4 | na | sing | 1:HIS35:NE2 | 2:ZN1:ZN |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 81.8 % (399 of 488) | 82.6 % (213 of 258) | 83.6 % (153 of 183) | 70.2 % (33 of 47) |
Backbone | 74.0 % (194 of 262) | 70.7 % (65 of 92) | 78.7 % (100 of 127) | 67.4 % (29 of 43) |
Sidechain | 89.8 % (238 of 265) | 89.2 % (148 of 166) | 90.5 % (86 of 95) | 100.0 % (4 of 4) |
Aromatic | 100.0 % (34 of 34) | 100.0 % (17 of 17) | 100.0 % (17 of 17) | |
Methyl | 100.0 % (24 of 24) | 100.0 % (12 of 12) | 100.0 % (12 of 12) |
1. zf-C2H2
GSSGSSGHTR KRYECSKCQA TFNLRKHLIQ HQKTHAAKSG PSSGSolvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | zf-C2H2 | [U-13C; U-15N] | protein | 1.0 mM |
2 | d-Tris-HCl | buffer | 20 mM | |
3 | NaCl | salt | 100 mM | |
4 | ZnCl2 | salt | 0.05 mM | |
5 | IDA | salt | 1 mM | |
6 | d-DTT | salt | 1 mM | |
7 | NaN3 | salt | 0.02 % | |
8 | H2O | solvent | 90 % | |
9 | D2O | solvent | 10 % |