Solution structure of the C2H2 type zinc finger (region 547-579) of human Zinc finger protein 484
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | na | sing | 1:CYS15:SG | 2:ZN1:ZN |
2 | na | sing | 1:CYS18:SG | 2:ZN1:ZN |
3 | na | sing | 1:HIS31:NE2 | 2:ZN1:ZN |
4 | na | sing | 1:HIS35:NE2 | 2:ZN1:ZN |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 88.5 % (430 of 486) | 88.7 % (228 of 257) | 90.7 % (166 of 183) | 78.3 % (36 of 46) |
Backbone | 84.2 % (229 of 272) | 83.7 % (82 of 98) | 86.9 % (113 of 130) | 77.3 % (34 of 44) |
Sidechain | 93.3 % (235 of 252) | 91.8 % (146 of 159) | 95.6 % (87 of 91) | 100.0 % (2 of 2) |
Aromatic | 100.0 % (30 of 30) | 100.0 % (15 of 15) | 100.0 % (15 of 15) | |
Methyl | 100.0 % (18 of 18) | 100.0 % (9 of 9) | 100.0 % (9 of 9) |
1. zf-C2H2 domain
GSSGSSGTGE RHYECSECGK AFIQKSTLSM HQRIHRGEKP SGPSSGSolvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | zf-C2H2 domain | [U-13C; U-15N] | protein | 1.0 (±0.1) mM |
2 | d-Tris-HCl | buffer | 20 mM | |
3 | NaCl | salt | 100 mM | |
4 | ZnCl2 | salt | 0.05 mM | |
5 | IDA | salt | 1 mM | |
6 | d-DTT | salt | 1 mM | |
7 | NaN3 | salt | 0.05 % | |
8 | H2O | solvent | 90 % | |
9 | D2O | solvent | 10 % |