Solution structure of the C2H2 type zinc finger (region 668-70) of human Zinc finger protein 28 homolog
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | na | sing | 1:CYS15:SG | 2:ZN1:ZN |
2 | na | sing | 1:CYS18:SG | 2:ZN1:ZN |
3 | na | sing | 1:HIS31:NE2 | 2:ZN1:ZN |
4 | na | sing | 1:HIS35:NE2 | 2:ZN1:ZN |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 88.7 % (430 of 485) | 88.6 % (226 of 255) | 90.8 % (167 of 184) | 80.4 % (37 of 46) |
Backbone | 84.1 % (227 of 270) | 82.5 % (80 of 97) | 86.9 % (113 of 130) | 79.1 % (34 of 43) |
Sidechain | 93.7 % (237 of 253) | 92.4 % (146 of 158) | 95.7 % (88 of 92) | 100.0 % (3 of 3) |
Aromatic | 100.0 % (30 of 30) | 100.0 % (15 of 15) | 100.0 % (15 of 15) | |
Methyl | 100.0 % (22 of 22) | 100.0 % (11 of 11) | 100.0 % (11 of 11) |
1. zf-C2H2, UNP residues 668-700
GSSGSSGTGE KPYECKECGK AFSQTTHLIQ HQRVHTGEKP SGPSSGSolvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | zf-C2H2, UNP residues 668-700 | [U-13C; U-15N] | protein | 1.0 (±0.1) mM |
2 | d-Tris-HCl | buffer | 20 mM | |
3 | NaCl | salt | 100 mM | |
4 | ZnCl2 | salt | 0.05 mM | |
5 | IDA | salt | 1 mM | |
6 | d-DTT | salt | 1 mM | |
7 | NaN3 | salt | 0.02 % | |
8 | D2O | solvent | 10 % | |
9 | H2O | solvent | 90 % |