Solution structure of the C2H2 type zinc finger (region 809-841) of human Zinc finger protein 473
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | na | sing | 1:CYS15:SG | 2:ZN1:ZN |
2 | na | sing | 1:CYS18:SG | 2:ZN1:ZN |
3 | na | sing | 1:HIS31:NE2 | 2:ZN1:ZN |
4 | na | sing | 1:HIS35:NE2 | 2:ZN1:ZN |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 82.9 % (394 of 475) | 83.6 % (204 of 244) | 84.2 % (154 of 183) | 75.0 % (36 of 48) |
Backbone | 76.1 % (207 of 272) | 74.2 % (72 of 97) | 78.6 % (103 of 131) | 72.7 % (32 of 44) |
Sidechain | 90.5 % (219 of 242) | 89.8 % (132 of 147) | 91.2 % (83 of 91) | 100.0 % (4 of 4) |
Aromatic | 100.0 % (30 of 30) | 100.0 % (15 of 15) | 100.0 % (15 of 15) | |
Methyl | 94.7 % (36 of 38) | 94.7 % (18 of 19) | 94.7 % (18 of 19) |
1. zf-C2H2 domain
GSSGSSGTGE KPYSCNVCGK AFVLSAHLNQ HLRVHTQETL SGPSSGSolvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | zf-C2H2 domain | [U-13C; U-15N] | protein | 1.0 (±0.1) mM |
2 | d-Tris-HCl | buffer | 20 mM | |
3 | NaCl | salt | 100 mM | |
4 | ZnCl2 | salt | 0.05 mM | |
5 | IDA | salt | 1 mM | |
6 | d-DTT | salt | 1 mM | |
7 | NaN3 | salt | 0.02 % | |
8 | H2O | solvent | 90 % | |
9 | D2O | solvent | 10 % |