Solution structure of the C2H2 type zinc finger (region 528-560) of human Zinc finger protein 28 homolog
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | na | sing | 1:CYS15:SG | 2:ZN1:ZN |
2 | na | sing | 1:CYS18:SG | 2:ZN1:ZN |
3 | na | sing | 1:HIS31:NE2 | 2:ZN1:ZN |
4 | na | sing | 1:HIS35:NE2 | 2:ZN1:ZN |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 83.5 % (405 of 485) | 84.6 % (215 of 254) | 84.0 % (157 of 187) | 75.0 % (33 of 44) |
Backbone | 75.6 % (204 of 270) | 74.2 % (72 of 97) | 76.9 % (100 of 130) | 74.4 % (32 of 43) |
Sidechain | 92.1 % (233 of 253) | 91.1 % (143 of 157) | 93.7 % (89 of 95) | 100.0 % (1 of 1) |
Aromatic | 100.0 % (38 of 38) | 100.0 % (19 of 19) | 100.0 % (19 of 19) | |
Methyl | 100.0 % (22 of 22) | 100.0 % (11 of 11) | 100.0 % (11 of 11) |
1. zf-C2H2 domain
GSSGSSGTGE KPYKCDVCHK SFRYGSSLTV HQRIHTGEKP SGPSSGSolvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | zf-C2H2 domain | [U-13C; U-15N] | protein | 1.0 (±0.1) mM |
2 | d-Tris-HCl | buffer | 20 mM | |
3 | NaCl | salt | 100 mM | |
4 | ZnCl2 | salt | 0.05 mM | |
5 | IDA | salt | 1 mM | |
6 | d-DTT | salt | 1 mM | |
7 | NaN3 | salt | 0.02 % | |
8 | H2O | solvent | 90 % | |
9 | D2O | solvent | 10 % |