BMRB: 10214

Solution structure of the second WW domain from the human membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1. MAGI-1

Authors

Ohnishi, S., Tochio, N., Tomizawa, T., Akasaka, R., Harada, T., Seki, E., Sato, M., Watanabe, S., Fujikura, Y., Koshiba, S., Terada, T., Shirouzu, M., Tanaka, A., Kigawa, T., Yokoyama, S.

Assembly

Membrane-associated guanylate kinase

Entity

1. Membrane-associated guanylate kinase (polymer, Thiol state: not present), 60 monomers, 6611.167 Da Detail

GSSGSSGLDS ELELPAGWEK IEDPVYGIYY VDHINRKTQY ENPVLEAKRK KQLESGPSSG

Formula weight

6611.167 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.0 %, Completeness: 92.3 %, Completeness (bb): 92.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.3 % (631 of 684)	89.4 % (322 of 360)	95.8 % (252 of 263)	93.4 % (57 of 61)
Backbone	92.0 % (324 of 352)	87.8 % (108 of 123)	94.8 % (164 of 173)	92.9 % (52 of 56)
Sidechain	93.0 % (358 of 385)	90.3 % (214 of 237)	97.2 % (139 of 143)	100.0 % (5 of 5)
Aromatic	100.0 % (48 of 48)	100.0 % (24 of 24)	100.0 % (23 of 23)	100.0 % (1 of 1)
Methyl	98.0 % (49 of 50)	96.0 % (24 of 25)	100.0 % (25 of 25)

1. WW domain

GSSGSSGLDS ELELPAGWEK IEDPVYGIYY VDHINRKTQY ENPVLEAKRK KQLESGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	WW domain	[U-13C; U-15N]	protein	1.0 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 mM
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.11 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.11 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.04 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2YSE, Strand ID: A Detail

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Release date

2009-04-03

Citation

Structural basis for controlling the dimerization and stability of the WW domains of an atypical subfamily
Ohnishi, S., Tochio, N., Tomizawa, T., Akasaka, R., Harada, T., Seki, E., Sato, M., Watanabe, S., Fujikura, Y., Koshiba, S., Terada, T., Shirouzu, M., Tanaka, A., Kigawa, T., Yokoyama, S.
Protein Sci. (2008), 17, 1531-1541, PubMed 18562638 , DOI 10.1110/ps.035329.108 , Abstract

Related entities 1. Membrane-associated guanylate kinase, : 1 : 41 entities Detail

Experiments performed 2 experiments Detail