Solution structure of the short-isoform of the second WW domain from the human membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 (MAGI-1)
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 92.7 % (497 of 536) | 91.8 % (256 of 279) | 94.7 % (197 of 208) | 89.8 % (44 of 49) |
Backbone | 91.3 % (261 of 286) | 90.1 % (91 of 101) | 92.9 % (130 of 140) | 88.9 % (40 of 45) |
Sidechain | 94.2 % (275 of 292) | 92.7 % (165 of 178) | 96.4 % (106 of 110) | 100.0 % (4 of 4) |
Aromatic | 100.0 % (48 of 48) | 100.0 % (24 of 24) | 100.0 % (23 of 23) | 100.0 % (1 of 1) |
Methyl | 100.0 % (36 of 36) | 100.0 % (18 of 18) | 100.0 % (18 of 18) |
1. WW domain1
GSSGSSGLDS ELELPAGWEK IEDPVYGIYY VDHINRKTQY ENPSGPSSGSolvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | WW domain1 | [U-13C; U-15N] | protein | 0.7 mM |
2 | d-Tris-HCl | buffer | 20 mM | |
3 | NaCl | salt | 100 mM | |
4 | d-DTT | salt | 1 mM | |
5 | NaN3 | salt | 0.02 % | |
6 | H2O | solvent | 90 % | |
7 | D2O | solvent | 10 % |