Solution structure of the C2H2 type zinc finger (region 411-441) of human Zinc finger protein 268
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | na | sing | 1:CYS15:SG | 2:ZN1:ZN |
2 | na | sing | 1:CYS18:SG | 2:ZN1:ZN |
3 | na | sing | 1:HIS31:NE2 | 2:ZN1:ZN |
4 | na | sing | 1:HIS35:NE2 | 2:ZN1:ZN |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 86.8 % (396 of 456) | 87.0 % (208 of 239) | 87.6 % (149 of 170) | 83.0 % (39 of 47) |
Backbone | 81.2 % (211 of 260) | 78.5 % (73 of 93) | 83.2 % (104 of 125) | 81.0 % (34 of 42) |
Sidechain | 93.1 % (217 of 233) | 92.5 % (135 of 146) | 93.9 % (77 of 82) | 100.0 % (5 of 5) |
Aromatic | 100.0 % (26 of 26) | 100.0 % (13 of 13) | 100.0 % (13 of 13) | |
Methyl | 100.0 % (18 of 18) | 100.0 % (9 of 9) | 100.0 % (9 of 9) |
1. zf-C2H2 domain
GSSGSSGTGE KPYECNECQK AFNTKSNLMV HQRTHTGESG PSSGSolvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | zf-C2H2 domain | [U-13C; U-15N] | protein | 1.0 (±0.1) mM |
2 | d-Tris-HCl | buffer | 20 mM | |
3 | NaCl | salt | 100 mM | |
4 | ZnCl2 | salt | 0.05 mM | |
5 | IDA | salt | 1 mM | |
6 | d-DTT | salt | 1 mM | |
7 | NaN3 | salt | 0.02 % | |
8 | H2O | solvent | 90 % | |
9 | D2O | solvent | 10 % |