BMRB: 10217

Solution structure of the C2H2 type zinc finger (region 693-723) of human Zinc finger protein 268

Authors

Tochio, N., Tomizawa, T., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.

Assembly

Zinc finger protein 268

Entity

1. zf-C2H2 domain (polymer, Thiol state: all other bound), 44 monomers, 4517.005 Da Detail

GSSGSSGVKP YGCSECGKAF RSKSYLIIHM RTHTGEKPSG PSSG

2. ZN (non-polymer), 65.409 Da

Total weight

4582.414 Da

Max. entity weight

4517.005 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS13:SG	2:ZN1:ZN
2	na	sing	1:CYS16:SG	2:ZN1:ZN
3	na	sing	1:HIS29:NE2	2:ZN1:ZN
4	na	sing	1:HIS33:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 79.5 %, Completeness: 84.3 %, Completeness (bb): 77.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	84.3 % (392 of 465)	84.4 % (206 of 244)	86.1 % (155 of 180)	75.6 % (31 of 41)
Backbone	77.9 % (201 of 258)	76.3 % (71 of 93)	79.8 % (99 of 124)	75.6 % (31 of 41)
Sidechain	90.9 % (221 of 243)	89.4 % (135 of 151)	93.5 % (86 of 92)
Aromatic	100.0 % (34 of 34)	100.0 % (17 of 17)	100.0 % (17 of 17)
Methyl	100.0 % (22 of 22)	100.0 % (11 of 11)	100.0 % (11 of 11)

1. zf-C2H2 domain

GSSGSSGVKP YGCSECGKAF RSKSYLIIHM RTHTGEKPSG PSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	zf-C2H2 domain	[U-13C; U-15N]	protein	1.0 (±0.1) mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	ZnCl2		salt	0.05 mM
5	IDA		salt	1 mM
6	d-DTT		salt	1 mM
7	NaN3		salt	0.02 %
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EOG, Strand ID: A Detail

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Release date

2009-05-27

Citation

Solution structure of the C2H2 type zinc finger (region 693-723) of human Zinc finger protein 268
Tochio, N., Tomizawa, T., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.

Related entities 1. zf-C2H2 domain, : 1 : 306 entities Detail

Experiments performed 2 experiments Detail