BMRB: 10222

Solution structure of the C2H2 type zinc finger (region 775-807) of human Zinc finger protein 268

Authors

Tochio, N., Tomizawa, T., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.

Assembly

Zinc finger protein 268

Entity

1. zf-C2H2 (polymer, Thiol state: all other bound), 46 monomers, 4591.984 Da Detail

GSSGSSGAGE KPYGCSECGK AFSSKSYLII HMRTHSGEKP SGPSSG

2. ZN (non-polymer), 65.409 Da

Total weight

4657.393 Da

Max. entity weight

4591.984 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS15:SG	2:ZN1:ZN
2	na	sing	1:CYS18:SG	2:ZN1:ZN
3	na	sing	1:HIS31:NE2	2:ZN1:ZN
4	na	sing	1:HIS35:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 87.0 %, Completeness: 84.3 %, Completeness (bb): 78.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	84.3 % (396 of 470)	85.4 % (210 of 246)	83.4 % (151 of 181)	81.4 % (35 of 43)
Backbone	78.5 % (212 of 270)	78.6 % (77 of 98)	77.5 % (100 of 129)	81.4 % (35 of 43)
Sidechain	90.3 % (214 of 237)	89.9 % (133 of 148)	91.0 % (81 of 89)
Aromatic	100.0 % (34 of 34)	100.0 % (17 of 17)	100.0 % (17 of 17)
Methyl	100.0 % (18 of 18)	100.0 % (9 of 9)	100.0 % (9 of 9)

1. zf-C2H2

GSSGSSGAGE KPYGCSECGK AFSSKSYLII HMRTHSGEKP SGPSSG

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	zf-C2H2	[U-13C; U-15N]	protein	1 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	ZnCl2		salt	0.05 mM
5	IDA		salt	1 mM
6	d-DTT		salt	1 mM
7	NaN3		salt	0.02 %
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2YTQ, Strand ID: A Detail

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Release date

2009-05-28

Citation

Solution structure of the C2H2 type zinc finger (region 775-807) of human Zinc finger protein 268
Tochio, N., Tomizawa, T., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.

Related entities 1. zf-C2H2, : 1 : 227 entities Detail

Experiments performed 2 experiments Detail