BMRB: 10226

Solution structure of the C2H2 type zinc finger (region 273-303) of human Zinc finger protein 268

Authors

Tochio, N., Tomizawa, T., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.

Assembly

Zinc finger protein 268

Entity

1. zf-C2H2, UNP residues 273-303 (polymer, Thiol state: free and disulfide bound), 44 monomers, 4504.856 Da Detail

GSSGSSGEKP FGCSCCEKAF SSKSYLLVHQ QTHAEEKPSG PSSG

2. ZN (non-polymer), 65.409 Da

Total weight

4570.265 Da

Max. entity weight

4504.856 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS13:SG	2:ZN1:ZN
2	na	sing	1:CYS16:SG	2:ZN1:ZN
3	na	sing	1:HIS29:NE2	2:ZN1:ZN
4	na	sing	1:HIS33:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 84.1 %, Completeness: 85.9 %, Completeness (bb): 78.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	85.9 % (397 of 462)	87.2 % (211 of 242)	85.9 % (152 of 177)	79.1 % (34 of 43)
Backbone	78.7 % (203 of 258)	78.0 % (71 of 91)	80.2 % (101 of 126)	75.6 % (31 of 41)
Sidechain	88.4 % (214 of 242)	87.4 % (132 of 151)	89.9 % (80 of 89)	100.0 % (2 of 2)
Aromatic	100.0 % (36 of 36)	100.0 % (18 of 18)	100.0 % (18 of 18)
Methyl	100.0 % (18 of 18)	100.0 % (9 of 9)	100.0 % (9 of 9)

1. zf-C2H2, UNP residues 273-303

GSSGSSGEKP FGCSCCEKAF SSKSYLLVHQ QTHAEEKPSG PSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	zf-C2H2, UNP residues 273-303	[U-13C; U-15N]	protein	1.0 (±0.1) mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	ZnCl2		salt	0.05 mM
5	IDA		salt	1 mM
6	d-DTT		salt	1 mM
7	NaN3		salt	0.02 %
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EMX, Strand ID: A Detail

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Release date

2009-08-18

Citation

Solution structure of the C2H2 type zinc finger (region 273-303) of human Zinc finger protein 268
Tochio, N., Tomizawa, T., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.

Related entities 1. zf-C2H2, UNP residues 273-303, : 1 : 297 entities Detail

Experiments performed 2 experiments Detail