BMRB: 10228

Solution structure of the C2H2 type zinc finger (region 628-660) of human Zinc finger protein 95 homolog

Authors

Li, H., Tochio, N., Tomizawa, T., Abe, H., Saito, K., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.

Assembly

Zinc finger protein 95 homolog

Entity

1. zf-C2H2 (polymer, Thiol state: all disulfide bound), 46 monomers, 4730.105 Da Detail

GSSGSSGSGE RPFKCNECGK GFGRRSHLAG HLRLHSREKS SGPSSG

2. ZN (non-polymer), 65.409 Da

Total weight

4795.514 Da

Max. entity weight

4730.105 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS15:SG	2:ZN1:ZN
2	na	sing	1:CYS18:SG	2:ZN1:ZN
3	na	sing	1:HIS31:NE2	2:ZN1:ZN
4	na	sing	1:HIS35:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 84.8 %, Completeness: 83.5 %, Completeness (bb): 77.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	83.5 % (400 of 479)	83.9 % (214 of 255)	83.8 % (150 of 179)	80.0 % (36 of 45)
Backbone	77.2 % (210 of 272)	76.0 % (76 of 100)	77.3 % (99 of 128)	79.5 % (35 of 44)
Sidechain	90.9 % (221 of 243)	89.0 % (138 of 155)	94.3 % (82 of 87)	100.0 % (1 of 1)
Aromatic	100.0 % (32 of 32)	100.0 % (16 of 16)	100.0 % (16 of 16)
Methyl	100.0 % (14 of 14)	100.0 % (7 of 7)	100.0 % (7 of 7)

1. zf-C2H2

GSSGSSGSGE RPFKCNECGK GFGRRSHLAG HLRLHSREKS SGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	zf-C2H2	[U-13C; U-15N]	protein	1.0 (±0.1) mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	ZnCl2		salt	0.05 mM
5	IDA		salt	1 mM
6	d-DTT		salt	1 mM
7	NaN3		salt	0.02 %
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EMZ, Strand ID: A Detail

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Release date

2009-08-18

Citation

Solution structure of the C2H2 type zinc finger (region 628-660) of human Zinc finger protein 95 homolog
Tochio, N., Tomizawa, T., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.

Related entities 1. zf-C2H2, : 1 : 148 entities Detail

Experiments performed 2 experiments Detail