BMRB: 10229

Solution structure of the C2H2 type zinc finger (region 385-413) of human Zinc finger protein 268

Authors

Tochio, N., Tomizawa, T., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.

Assembly

Zinc finger protein 268

Entity

1. zf-C2H2 (polymer, Thiol state: all disulfide bound), 42 monomers, 4291.694 Da Detail

GSSGSSGQKP YVCNECGKAF GLKSQLIIHE RIHTGESGPS SG

2. ZN (non-polymer), 65.409 Da

Total weight

4357.103 Da

Max. entity weight

4291.694 Da

Entity Connection

na 4 Detail

---

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS13:SG	2:ZN1:ZN
2	na	sing	1:CYS16:SG	2:ZN1:ZN
3	na	sing	1:HIS29:NE2	2:ZN1:ZN
4	na	sing	1:HIS33:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 83.3 %, Completeness: 85.6 %, Completeness (bb): 80.2 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	85.6 % (381 of 445)	84.1 % (196 of 233)	88.8 % (150 of 169)	81.4 % (35 of 43)
Backbone	80.2 % (199 of 248)	75.6 % (68 of 90)	83.9 % (99 of 118)	80.0 % (32 of 40)
Sidechain	91.3 % (211 of 231)	89.5 % (128 of 143)	94.1 % (80 of 85)	100.0 % (3 of 3)
Aromatic	100.0 % (26 of 26)	100.0 % (13 of 13)	100.0 % (13 of 13)
Methyl	100.0 % (28 of 28)	100.0 % (14 of 14)	100.0 % (14 of 14)

1. zf-C2H2

GSSGSSGQKP YVCNECGKAF GLKSQLIIHE RIHTGESGPS SG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	zf-C2H2	[U-13C; U-15N]	protein	1.0 (±0.1) mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	ZnCl2		salt	0.05 mM
5	IDA		salt	1 mM
6	d-DTT		salt	1 mM
7	NaN3		salt	0.02 %
8	H2O		solvent	90 %
9	D2O		solvent	10 %

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 2EN0, Strand ID: A Detail

---

Release date

2009-08-18

Citation

Solution structure of the C2H2 type zinc finger (region 385-413) of human Zinc finger protein 268
Tochio, N., Tomizawa, T., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.

Related entities 1. zf-C2H2, : 1 : 159 entities Detail

Experiments performed 2 experiments Detail