BMRB: 10231

Solution structure of the C2H2 type zinc finger (region 598-626) of human B-cell lymphoma 6 protein

Authors

Tochio, N., Tomizawa, T., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.

Assembly

B-cell lymphoma 6 protein

Entity

1. zf-C2H2, UNP residues 598-626 (polymer, Thiol state: all disulfide bound), 42 monomers, 4213.629 Da Detail

GSSGSSGGEK PYKCETCGAR FVQVAHLRAH VLIHTGSGPS SG

2. ZN (non-polymer), 65.409 Da

Total weight

4279.038 Da

Max. entity weight

4213.629 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS14:SG	2:ZN1:ZN
2	na	sing	1:CYS17:SG	2:ZN1:ZN
3	na	sing	1:HIS30:NE2	2:ZN1:ZN
4	na	sing	1:HIS34:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 81.0 %, Completeness: 85.3 %, Completeness (bb): 80.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	85.3 % (365 of 428)	84.5 % (186 of 220)	87.4 % (146 of 167)	80.5 % (33 of 41)
Backbone	80.2 % (199 of 248)	77.8 % (70 of 90)	82.2 % (97 of 118)	80.0 % (32 of 40)
Sidechain	91.1 % (195 of 214)	89.2 % (116 of 130)	94.0 % (78 of 83)	100.0 % (1 of 1)
Aromatic	100.0 % (30 of 30)	100.0 % (15 of 15)	100.0 % (15 of 15)
Methyl	100.0 % (34 of 34)	100.0 % (17 of 17)	100.0 % (17 of 17)

1. zf-C2H2, UNP residues 598-626

GSSGSSGGEK PYKCETCGAR FVQVAHLRAH VLIHTGSGPS SG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	zf-C2H2, UNP residues 598-626	[U-13C; U-15N]	protein	1.0 (±0.1) mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	ZnCl2		salt	0.05 mM
5	IDA		salt	1 mM
6	d-DTT		salt	1 mM
7	NaN3		salt	0.02 %
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.23 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.23 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.28 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EN2, Strand ID: A Detail

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Release date

2009-08-18

Citation

Solution structure of the C2H2 type zinc finger (region 598-626) of human B-cell lymphoma 6 protein
Tochio, N., Tomizawa, T., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.

Related entities 1. zf-C2H2, UNP residues 598-626, : 1 : 219 entities Detail

Experiments performed 2 experiments Detail