BMRB: 10240

Solution structures of the SH3 domain of human Src substrate cortactin

Authors

Sato, M., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

cortactin isoform a

Entity

1. cortactin isoform a (polymer, Thiol state: all free), 79 monomers, 8593.107 Da Detail

GSSGSSGTYD EYENDLGITA VALYDYQAAG DDEISFDPDD IITNIEMIDD GWWRGVCKGR YGLFPANYVE LRQSGPSSG

Formula weight

8593.107 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.5 %, Completeness: 96.4 %, Completeness (bb): 95.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.4 % (821 of 852)	95.6 % (417 of 436)	97.9 % (326 of 333)	94.0 % (78 of 83)
Backbone	95.3 % (446 of 468)	94.0 % (156 of 166)	96.9 % (219 of 226)	93.4 % (71 of 76)
Sidechain	97.6 % (441 of 452)	96.7 % (261 of 270)	98.9 % (173 of 175)	100.0 % (7 of 7)
Aromatic	100.0 % (92 of 92)	100.0 % (46 of 46)	100.0 % (44 of 44)	100.0 % (2 of 2)
Methyl	100.0 % (68 of 68)	100.0 % (34 of 34)	100.0 % (34 of 34)

1. SH3 domain

GSSGSSGTYD EYENDLGITA VALYDYQAAG DDEISFDPDD IITNIEMIDD GWWRGVCKGR YGLFPANYVE LRQSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain	[U-13C; U-15N]	protein	1 mM
2	d-Tris HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 2.2 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 2.2 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 3.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1X69, Strand ID: A Detail

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Release date

2009-11-02

Citation

Solution structures of the SH3 domain of human Src substrate cortactin
Sato, M., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. cortactin isoform a, : 1 : 2 : 58 entities Detail

Experiments performed 2 experiments Detail