BMRB: 10241

Solution structures of the SH3 domain of human rho guanine exchange factor (GEF) 16

Authors

Sato, M., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Rho guanine exchange factor (GEF) 16

Entity

1. Rho guanine exchange factor (GEF) 16 (polymer, Thiol state: not present), 79 monomers, 8566.255 Da Detail

GSSGSSGWQG LSSKGDLPQV EITKAFFAKQ ADEVTLQQAD VVLVLQQEDG WLYGERLRDG ETGWFPEDFA RFISGPSSG

Formula weight

8566.255 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.4 %, Completeness: 92.5 %, Completeness (bb): 89.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.5 % (824 of 891)	92.4 % (426 of 461)	93.0 % (320 of 344)	90.7 % (78 of 86)
Backbone	89.1 % (417 of 468)	88.0 % (146 of 166)	89.8 % (203 of 226)	89.5 % (68 of 76)
Sidechain	95.7 % (470 of 491)	94.9 % (280 of 295)	96.8 % (180 of 186)	100.0 % (10 of 10)
Aromatic	97.9 % (92 of 94)	97.9 % (46 of 47)	97.7 % (43 of 44)	100.0 % (3 of 3)
Methyl	100.0 % (72 of 72)	100.0 % (36 of 36)	100.0 % (36 of 36)

1. SH3 domain

GSSGSSGWQG LSSKGDLPQV EITKAFFAKQ ADEVTLQQAD VVLVLQQEDG WLYGERLRDG ETGWFPEDFA RFISGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain	[U-13C; U-15N]	protein	1 mM
2	d-Tris HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.16 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.16 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.42 ppm, Outliers: 5 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1X6B, Strand ID: A Detail

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Release date

2009-11-02

Citation

Solution structures of the SH3 domain of human rho guanine exchange factor (GEF) 16
Sato, M., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Rho guanine exchange factor (GEF) 16, : 1 : 63 entities Detail

Interaction partners 1. Rho guanine exchange factor (GEF) 16, : 6 interactors Detail

Experiments performed 2 experiments Detail