BMRB: 10243

Solution structures of the C2H2 type zinc finger domain of human Zinc finger protein 24

Authors

Sato, M., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Zinc finger protein 24

Entity

1. C2H2 type zinc finger domain (polymer, Thiol state: all other bound), 72 monomers, 7588.349 Da Detail

GSSGSSGIHS GEKPYGCVEC GKAFSRSSIL VQHQRVHTGE KPYKCLECGK AFSQNSGLIN HQRIHTSGPS SG

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

7719.167 Da

Max. entity weight

7588.349 Da

Entity Connection

na 8 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS17:SG	2:ZN1:ZN
2	na	sing	1:CYS20:SG	2:ZN1:ZN
3	na	sing	1:HIS33:NE2	2:ZN1:ZN
4	na	sing	1:HIS37:NE2	2:ZN1:ZN
5	na	sing	1:CYS45:SG	2:ZN1:ZN
6	na	sing	1:CYS48:SG	2:ZN1:ZN
7	na	sing	1:HIS61:NE2	2:ZN1:ZN
8	na	sing	1:HIS65:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.3 %, Completeness: 88.9 %, Completeness (bb): 85.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	88.9 % (694 of 781)	88.8 % (363 of 409)	89.9 % (267 of 297)	85.3 % (64 of 75)
Backbone	85.4 % (364 of 426)	84.2 % (128 of 152)	86.8 % (178 of 205)	84.1 % (58 of 69)
Sidechain	92.3 % (384 of 416)	91.4 % (235 of 257)	93.5 % (143 of 153)	100.0 % (6 of 6)
Aromatic	89.3 % (50 of 56)	89.3 % (25 of 28)	89.3 % (25 of 28)
Methyl	97.9 % (47 of 48)	95.8 % (23 of 24)	100.0 % (24 of 24)

1. C2H2 type zinc finger domain

GSSGSSGIHS GEKPYGCVEC GKAFSRSSIL VQHQRVHTGE KPYKCLECGK AFSQNSGLIN HQRIHTSGPS SG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	C2H2 type zinc finger domain	[U-13C; U-15N]	protein	1 mM
2	d-Tris HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	5 mM
5	NaN3		salt	0.02 %
6	ZnCl2		salt	0.01 mM
7	H2O		solvent	90 %
8	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.16 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.16 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.12 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1X6E, Strand ID: A Detail

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Release date

2009-11-02

Citation

Solution structures of the C2H2 type zinc finger domain of human Zinc finger protein 24
Sato, M., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. C2H2 type zinc finger domain, : 1 : 9 : 2087 entities Detail

Interaction partners 1. C2H2 type zinc finger domain, : 1 interactors Detail

Experiments performed 2 experiments Detail