BMRB: 10244

Solution structures of the C2H2 type zinc finger domain of human Zinc finger protein 462

Authors

Sato, M., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Zinc finger protein 462

Entity

1. C2H2 type zinc finger domain (polymer, Thiol state: all other bound), 88 monomers, 9827.791 Da Detail

GSSGSSGLKR DFIILGNGPR LQNSTYQCKH CDSKLQSTAE LTSHLNIHNE EFQKRAKRQE RRKQLLSKQK YADGAFADFK QESGPSSG

2. ZN (non-polymer), 65.409 Da

Total weight

9893.2 Da

Max. entity weight

9827.791 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS28:SG	2:ZN1:ZN
2	na	sing	1:CYS31:SG	2:ZN1:ZN
3	na	sing	1:HIS44:NE2	2:ZN1:ZN
4	na	sing	1:HIS48:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.6 %, Completeness: 82.4 %, Completeness (bb): 82.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	82.4 % (845 of 1026)	80.8 % (441 of 546)	84.8 % (324 of 382)	81.6 % (80 of 98)
Backbone	82.3 % (431 of 524)	80.2 % (146 of 182)	84.0 % (215 of 256)	81.4 % (70 of 86)
Sidechain	81.3 % (473 of 582)	79.7 % (290 of 364)	84.5 % (174 of 206)	75.0 % (9 of 12)
Aromatic	73.5 % (50 of 68)	79.4 % (27 of 34)	67.6 % (23 of 34)
Methyl	93.3 % (56 of 60)	93.3 % (28 of 30)	93.3 % (28 of 30)

1. C2H2 type zinc finger domain

GSSGSSGLKR DFIILGNGPR LQNSTYQCKH CDSKLQSTAE LTSHLNIHNE EFQKRAKRQE RRKQLLSKQK YADGAFADFK QESGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	C2H2 type zinc finger domain	[U-13C; U-15N]	protein	1 mM
2	d-Tris HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	ZnCl2		salt	50 uM
7	NTA		salt	0.1 mM
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.15 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.15 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.04 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.16 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1X6F, Strand ID: A Detail

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Release date

2009-11-02

Citation

Solution structures of the C2H2 type zinc finger domain of human Zinc finger protein 462
Sato, M., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. C2H2 type zinc finger domain, : 1 : 2 : 2 entities Detail

Interaction partners 1. C2H2 type zinc finger domain, : 9 interactors Detail

Experiments performed 2 experiments Detail