BMRB: 10245

Solution structures of the SH3 domain of human megakaryocyte-associated tyrosine-protein kinase.

Authors

Sato, M., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Megakaryocyte-associated tyrosine-protein kinase

Entity

1. Megakaryocyte-associated tyrosine-protein kinase (polymer, Thiol state: all free), 81 monomers, 8722.725 Da Detail

GSSGSSGRMP TRRWAPGTQC ITKCEHTRPK PGELAFRKGD VVTILEACEN KSWYRVKHHT SGQEGLLAAG ALRERSGPSS G

Formula weight

8722.725 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.4 %, Completeness: 92.0 %, Completeness (bb): 89.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.0 % (819 of 890)	91.7 % (429 of 468)	93.0 % (317 of 341)	90.1 % (73 of 81)
Backbone	89.7 % (427 of 476)	89.3 % (150 of 168)	90.1 % (209 of 232)	89.5 % (68 of 76)
Sidechain	94.2 % (456 of 484)	93.0 % (279 of 300)	96.1 % (172 of 179)	100.0 % (5 of 5)
Aromatic	96.3 % (52 of 54)	96.3 % (26 of 27)	96.0 % (24 of 25)	100.0 % (2 of 2)
Methyl	100.0 % (64 of 64)	100.0 % (32 of 32)	100.0 % (32 of 32)

1. SH3 domain

GSSGSSGRMP TRRWAPGTQC ITKCEHTRPK PGELAFRKGD VVTILEACEN KSWYRVKHHT SGQEGLLAAG ALRERSGPSS G

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain	[U-13C; U-15N]	protein	1 mM
2	d-Tris HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.02 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.02 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.01 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.38 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1X6G, Strand ID: A Detail

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Release date

2009-11-02

Citation

Solution structures of the SH3 domain of human megakaryocyte-associated tyrosine-protein kinase
Sato, M., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Megakaryocyte-associated tyrosine-protein kinase, : 1 : 51 entities Detail

Interaction partners 1. Megakaryocyte-associated tyrosine-protein kinase, : 10 interactors Detail

Experiments performed 2 experiments Detail