BMRB: 10246

Solution structures of the C2H2 type zinc finger domain of human Transcriptional repressor CTCF

Authors

Sato, M., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Transcriptional repressor CTCF

Entity

1. C2H2 type zinc finger domain (polymer, Thiol state: all other bound), 86 monomers, 9322.210 Da Detail

GSSGSSGRTH TGEKPYACSH CDKTFRQKQL LDMHFKRYHD PNFVPAAFVC SKCGKTFTRR NTMARHADNC AGPDGVEGEN SGPSSG

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

9453.028 Da

Max. entity weight

9322.21 Da

Entity Connection

na 8 Detail

---

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS18:SG	2:ZN1:ZN
2	na	sing	1:CYS21:SG	2:ZN1:ZN
3	na	sing	1:HIS34:NE2	2:ZN1:ZN
4	na	sing	1:HIS39:NE2	2:ZN1:ZN
5	na	sing	1:CYS50:SG	2:ZN1:ZN
6	na	sing	1:CYS53:SG	2:ZN1:ZN
7	na	sing	1:HIS66:NE2	2:ZN1:ZN
8	na	sing	1:CYS70:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.0 %, Completeness: 90.7 %, Completeness (bb): 86.0 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.7 % (847 of 934)	91.2 % (446 of 489)	91.9 % (329 of 358)	82.8 % (72 of 87)
Backbone	86.0 % (435 of 506)	84.2 % (149 of 177)	88.3 % (219 of 248)	82.7 % (67 of 81)
Sidechain	96.2 % (485 of 504)	95.2 % (297 of 312)	98.4 % (183 of 186)	83.3 % (5 of 6)
Aromatic	100.0 % (86 of 86)	100.0 % (43 of 43)	100.0 % (43 of 43)
Methyl	100.0 % (44 of 44)	100.0 % (22 of 22)	100.0 % (22 of 22)

1. C2H2 type zinc finger domain

GSSGSSGRTH TGEKPYACSH CDKTFRQKQL LDMHFKRYHD PNFVPAAFVC SKCGKTFTRR NTMARHADNC AGPDGVEGEN SGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	C2H2 type zinc finger domain	[U-13C; U-15N]	protein	1.5 mM
2	d-Tris HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	5 mM
5	NaN3		salt	0.02 %
6	ZnCl2		salt	10 uM
7	H2O		solvent	90 %
8	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.33 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.33 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: 0.01 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: -0.07 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 1X6H, Strand ID: A Detail

---

Release date

2009-11-02

Citation

Solution structures of the C2H2 type zinc finger domain of human Transcriptional repressor CTCF
Sato, M., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. C2H2 type zinc finger domain, : 1 : 8 : 332 entities Detail

Interaction partners 1. C2H2 type zinc finger domain, : 12 interactors Detail

Experiments performed 2 experiments Detail