BMRB: 10248

Solution Structure of the HMG_box Domain of Murine Bobby Sox Homolog

Authors

Li, H., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

HMG-BOX transcription factor BBX

Entity

1. HMG-BOX transcription factor BBX (polymer, Thiol state: all free), 82 monomers, 9271.442 Da Detail

GSSGSSGARR PMNAFLLFCK RHRSLVRQEH PRLDNRGATK ILADWWAVLD PKEKQKYTDM AKEYKDAFMK ANPGYRSGPS SG

Formula weight

9271.442 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.5 %, Completeness: 90.1 %, Completeness (bb): 87.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.1 % (868 of 963)	89.4 % (457 of 511)	92.1 % (339 of 368)	85.7 % (72 of 84)
Backbone	87.8 % (423 of 482)	86.7 % (144 of 166)	89.5 % (214 of 239)	84.4 % (65 of 77)
Sidechain	92.6 % (515 of 556)	90.7 % (313 of 345)	95.6 % (195 of 204)	100.0 % (7 of 7)
Aromatic	90.7 % (78 of 86)	90.7 % (39 of 43)	90.2 % (37 of 41)	100.0 % (2 of 2)
Methyl	100.0 % (56 of 56)	100.0 % (28 of 28)	100.0 % (28 of 28)

1. HMG box domain

GSSGSSGARR PMNAFLLFCK RHRSLVRQEH PRLDNRGATK ILADWWAVLD PKEKQKYTDM AKEYKDAFMK ANPGYRSGPS SG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	HMG_box domain	[U-13C; U-15N]	protein	1.55 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.33 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.33 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.06 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WZ6, Strand ID: A Detail

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Release date

2009-11-19

Citation

Solution Structure of the HMG_box Domain of Murine Bobby Sox Homolog
Li, H., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. HMG-BOX transcription factor BBX, : 1 : 276 entities Detail

Interaction partners 1. HMG-BOX transcription factor BBX, : 4 interactors Detail

Experiments performed 2 experiments Detail