Solution structure of the C-terminal PH domain of human pleckstrin
GSSGSSGVIL KEEFRGVIIK QGCLLKQGHR RKNWKVRKFI LREDPAYLHY YDPAGAEDPL GAIHLRGCVV TSVESNSNGR KSEEENLFEI ITADEVHYFL QAATPKERTE WIKAIQMASR TGKSGPSSG
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 94.6 % (1413 of 1493) | 93.9 % (734 of 782) | 96.2 % (555 of 577) | 92.5 % (124 of 134) |
Backbone | 93.1 % (711 of 764) | 91.7 % (244 of 266) | 94.4 % (353 of 374) | 91.9 % (114 of 124) |
Sidechain | 96.3 % (814 of 845) | 95.0 % (490 of 516) | 98.4 % (314 of 319) | 100.0 % (10 of 10) |
Aromatic | 98.2 % (110 of 112) | 98.2 % (55 of 56) | 98.1 % (53 of 54) | 100.0 % (2 of 2) |
Methyl | 100.0 % (128 of 128) | 100.0 % (64 of 64) | 100.0 % (64 of 64) |
1. PH domain
GSSGSSGVIL KEEFRGVIIK QGCLLKQGHR RKNWKVRKFI LREDPAYLHY YDPAGAEDPL GAIHLRGCVV TSVESNSNGR KSEEENLFEI ITADEVHYFL QAATPKERTE WIKAIQMASR TGKSGPSSGSolvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | PH domain | [U-13C; U-15N] | protein | 1.13 mM |
2 | d-Tris-HCl | buffer | 20 mM | |
3 | NaCl | salt | 100 mM | |
4 | d-DTT | salt | 1 mM | |
5 | NaN3 | salt | 0.02 % | |
6 | H2O | solvent | 90 % | |
7 | D2O | solvent | 10 % |