BMRB: 10249

Solution structure of the C-terminal PH domain of human pleckstrin

Authors

Li, H., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Pleckstrin

Entity

1. Pleckstrin (polymer, Thiol state: all free), 129 monomers, 14332.04 Da Detail

GSSGSSGVIL KEEFRGVIIK QGCLLKQGHR RKNWKVRKFI LREDPAYLHY YDPAGAEDPL GAIHLRGCVV TSVESNSNGR KSEEENLFEI ITADEVHYFL QAATPKERTE WIKAIQMASR TGKSGPSSG

Formula weight

14332.04 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.3 %, Completeness: 94.6 %, Completeness (bb): 93.1 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.6 % (1413 of 1493)	93.9 % (734 of 782)	96.2 % (555 of 577)	92.5 % (124 of 134)
Backbone	93.1 % (711 of 764)	91.7 % (244 of 266)	94.4 % (353 of 374)	91.9 % (114 of 124)
Sidechain	96.3 % (814 of 845)	95.0 % (490 of 516)	98.4 % (314 of 319)	100.0 % (10 of 10)
Aromatic	98.2 % (110 of 112)	98.2 % (55 of 56)	98.1 % (53 of 54)	100.0 % (2 of 2)
Methyl	100.0 % (128 of 128)	100.0 % (64 of 64)	100.0 % (64 of 64)

1. PH domain

GSSGSSGVIL KEEFRGVIIK QGCLLKQGHR RKNWKVRKFI LREDPAYLHY YDPAGAEDPL GAIHLRGCVV TSVESNSNGR KSEEENLFEI ITADEVHYFL QAATPKERTE WIKAIQMASR TGKSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	PH domain	[U-13C; U-15N]	protein	1.13 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.14 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.14 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.13 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 1X05, Strand ID: A Detail

---

Release date

2009-11-19

Citation

Solution structure of the C-terminal PH domain of human pleckstrin
Li, H., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Pleckstrin, : 1 : 4 : 113 entities Detail

Interaction partners 1. Pleckstrin, : 6 interactors Detail

Experiments performed 2 experiments Detail