BMRB: 10250

Solution structure of the PH domain of human Docking protein BRDG1

Authors

Li, H., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Signal-transducing adaptor protein 1

Entity

1. Signal-transducing adaptor protein 1 (polymer, Thiol state: all free), 149 monomers, 17051.17 Da Detail

GSSGSSGQER LKITALPLYF EGFLLIKRSG YREYEHYWTE LRGTTLFFYT DKKSIIYVDK LDIVDLTCLT EQNSTEKNCA KFTLVLPKEE VQLKTENTES GEEWRGFILT VTELSVPQNV SLLPGQVIKL HEVLEREKKR RIESGPSSG

Formula weight

17051.17 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.6 %, Completeness: 95.1 %, Completeness (bb): 92.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.1 % (1702 of 1790)	95.2 % (889 of 934)	96.0 % (673 of 701)	90.3 % (140 of 155)
Backbone	92.1 % (814 of 884)	91.1 % (277 of 304)	93.6 % (408 of 436)	89.6 % (129 of 144)
Sidechain	97.9 % (1022 of 1044)	97.1 % (612 of 630)	99.0 % (399 of 403)	100.0 % (11 of 11)
Aromatic	100.0 % (140 of 140)	100.0 % (70 of 70)	100.0 % (68 of 68)	100.0 % (2 of 2)
Methyl	99.4 % (173 of 174)	98.9 % (86 of 87)	100.0 % (87 of 87)

1. PH domain

GSSGSSGQER LKITALPLYF EGFLLIKRSG YREYEHYWTE LRGTTLFFYT DKKSIIYVDK LDIVDLTCLT EQNSTEKNCA KFTLVLPKEE VQLKTENTES GEEWRGFILT VTELSVPQNV SLLPGQVIKL HEVLEREKKR RIESGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	PH domain	[U-13C; U-15N]	protein	1.00 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.0 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.0 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.09 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.28 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1X1F, Strand ID: A Detail

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Release date

2009-11-19

Citation

Solution structure of the PH domain of human Docking protein BRDG1
Li, H., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Signal-transducing adaptor protein 1, : 1 : 19 entities Detail

Interaction partners 1. Signal-transducing adaptor protein 1, : 8 interactors Detail

Experiments performed 2 experiments Detail