BMRB: 10251

Solution structure of the C-terminal PH domain of human pleckstrin 2

Authors

Li, H., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Pleckstrin 2

Entity

1. Pleckstrin 2 (polymer, Thiol state: not present), 129 monomers, 14041.62 Da Detail

GSSGSSGSLS TVELSGTVVK QGYLAKQGHK RKNWKVRRFV LRKDPAFLHY YDPSKEENRP VGGFSLRGSL VSALEDNGVP TGVKGNVQGN LFKVITKDDT HYYIQASSKA ERAEWIEAIK KLTSGPSSG

Formula weight

14041.62 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.9 %, Completeness: 95.3 %, Completeness (bb): 93.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.3 % (1410 of 1480)	94.7 % (732 of 773)	96.9 % (554 of 572)	91.9 % (124 of 135)
Backbone	93.6 % (715 of 764)	92.5 % (248 of 268)	95.2 % (354 of 372)	91.1 % (113 of 124)
Sidechain	97.1 % (806 of 830)	95.8 % (484 of 505)	99.0 % (311 of 314)	100.0 % (11 of 11)
Aromatic	100.0 % (116 of 116)	100.0 % (58 of 58)	100.0 % (56 of 56)	100.0 % (2 of 2)
Methyl	100.0 % (126 of 126)	100.0 % (63 of 63)	100.0 % (63 of 63)

1. PH domain

GSSGSSGSLS TVELSGTVVK QGYLAKQGHK RKNWKVRRFV LRKDPAFLHY YDPSKEENRP VGGFSLRGSL VSALEDNGVP TGVKGNVQGN LFKVITKDDT HYYIQASSKA ERAEWIEAIK KLTSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	PH domain	[U-13C; U-15N]	protein	1.24 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.12 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.12 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.1 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1X1G, Strand ID: A Detail

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Release date

2009-11-19

Citation

Solution structure of the C-terminal PH domain of human pleckstrin 2
Li, H., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Pleckstrin 2, : 1 : 2 : 219 entities Detail

Interaction partners 1. Pleckstrin 2, : 4 interactors Detail

Experiments performed 2 experiments Detail