BMRB: 10252

Solution structure of the HMG_box domain of thymus high mobility group box protein TOX from mouse

Authors

Li, H., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

thymus high mobility group box protein TOX

Entity

1. thymus high mobility group box protein TOX (polymer, Thiol state: not present), 102 monomers, 11035.29 Da Detail

GSSGSSGKKK KKKDPNEPQK PVSAYALFFR DTQAAIKGQN PNATFGEVSK IVASMWDGLG EEQKQVYKKK TEAAKKEYLK QLAAYRASLV SKSYTDSGPS SG

Formula weight

11035.29 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.2 %, Completeness: 86.3 %, Completeness (bb): 86.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	86.3 % (1023 of 1185)	84.2 % (528 of 627)	88.9 % (401 of 451)	87.9 % (94 of 107)
Backbone	86.2 % (519 of 602)	84.6 % (176 of 208)	87.2 % (259 of 297)	86.6 % (84 of 97)
Sidechain	87.0 % (588 of 676)	84.0 % (352 of 419)	91.5 % (226 of 247)	100.0 % (10 of 10)
Aromatic	92.7 % (76 of 82)	92.7 % (38 of 41)	92.5 % (37 of 40)	100.0 % (1 of 1)
Methyl	98.7 % (77 of 78)	97.4 % (38 of 39)	100.0 % (39 of 39)

1. HMG box

GSSGSSGKKK KKKDPNEPQK PVSAYALFFR DTQAAIKGQN PNATFGEVSK IVASMWDGLG EEQKQVYKKK TEAAKKEYLK QLAAYRASLV SKSYTDSGPS SG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	HMG_box domain	[U-13C; U-15N]	protein	1.28 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.14 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.14 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.12 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2CO9, Strand ID: A Detail

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Release date

2009-11-19

Citation

Solution structure of the HMG_box domain of thymus high mobility group box protein TOX from mouse
Li, H., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. thymus high mobility group box protein TOX, : 1 : 3 : 63 entities Detail

Experiments performed 2 experiments Detail