BMRB: 10254

Solution structure of the C-terminal PH domain of FYVE, RhoGEF and PH domain containing protein 3 (FGD3) from human

Authors

Li, H., Tomizawa, T., Tochio, N., Muto, Y., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

FYVE, RhoGEF and PH domain containing protein 3

Entity

1. FYVE, RhoGEF and PH domain containing protein 3 (polymer, Thiol state: all free), 112 monomers, 11998.18 Da Detail

GSSGSSGSLL CGPLRLSESG ETWSEVWAAI PMSDPQVLHL QGGSQDGRLP RTIPLPSCKL SVPDPEERLD SGHVWKLQWA KQSWYLSASS AELQQQWLET LSTAAHSGPS SG

Formula weight

11998.18 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.8 %, Completeness: 94.0 %, Completeness (bb): 91.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.0 % (1168 of 1242)	93.3 % (602 of 645)	95.2 % (457 of 480)	93.2 % (109 of 117)
Backbone	91.4 % (598 of 654)	88.9 % (201 of 226)	92.9 % (302 of 325)	92.2 % (95 of 103)
Sidechain	96.7 % (666 of 689)	95.7 % (401 of 419)	98.0 % (251 of 256)	100.0 % (14 of 14)
Aromatic	100.0 % (92 of 92)	100.0 % (46 of 46)	100.0 % (40 of 40)	100.0 % (6 of 6)
Methyl	100.0 % (106 of 106)	100.0 % (53 of 53)	100.0 % (53 of 53)

1. PH domain

GSSGSSGSLL CGPLRLSESG ETWSEVWAAI PMSDPQVLHL QGGSQDGRLP RTIPLPSCKL SVPDPEERLD SGHVWKLQWA KQSWYLSASS AELQQQWLET LSTAAHSGPS SG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	PH domain	[U-13C; U-15N]	protein	1.20 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.2 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.2 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.2 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2COC, Strand ID: A Detail

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Release date

2009-11-19

Citation

Solution structure of the C-terminal PH domain of FYVE, RhoGEF and PH domain containing protein 3 (FGD3) from human
Li, H., Tomizawa, T., Tochio, N., Muto, Y., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. FYVE, RhoGEF and PH domain containing protein 3, : 1 : 2 : 46 entities Detail

Interaction partners 1. FYVE, RhoGEF and PH domain containing protein 3, : 1 interactors Detail

Experiments performed 2 experiments Detail