BMRB: 10255

Solution structure of the N-terminal PH domain of ARAP2 protein from human

Authors

Li, H., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

ARAP2 protein

Entity

1. ARAP2 protein (polymer, Thiol state: all free), 115 monomers, 12855.34 Da Detail

GSSGSSGKVK SGWLDKLSPQ GKRMFQKRWV KFDGLSISYY NNEKEMYSKG IIPLSAISTV RVQGDNKFEV VTTQRTFVFR VEKEEERNDW ISILLNALKS QSLTSQSQAS GPSSG

Formula weight

12855.34 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.4 %, Completeness: 88.1 %, Completeness (bb): 85.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	88.1 % (1199 of 1361)	88.0 % (629 of 715)	89.6 % (465 of 519)	82.7 % (105 of 127)
Backbone	85.8 % (587 of 684)	86.5 % (205 of 237)	86.0 % (288 of 335)	83.9 % (94 of 112)
Sidechain	90.4 % (707 of 782)	88.7 % (424 of 478)	94.1 % (272 of 289)	73.3 % (11 of 15)
Aromatic	96.4 % (106 of 110)	96.4 % (53 of 55)	98.1 % (51 of 52)	66.7 % (2 of 3)
Methyl	100.0 % (104 of 104)	100.0 % (52 of 52)	100.0 % (52 of 52)

1. PH domain

GSSGSSGKVK SGWLDKLSPQ GKRMFQKRWV KFDGLSISYY NNEKEMYSKG IIPLSAISTV RVQGDNKFEV VTTQRTFVFR VEKEEERNDW ISILLNALKS QSLTSQSQAS GPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	PH domain	[U-13C; U-15N]	protein	0.62 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.11 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.11 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.08 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.07 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2COD, Strand ID: A Detail

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Release date

2009-11-19

Citation

Solution structure of the N-terminal PH domain of ARAP2 protein from human
Li, H., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. ARAP2 protein, : 1 : 139 entities Detail

Interaction partners 1. ARAP2 protein, : 3 interactors Detail

Experiments performed 2 experiments Detail