BMRB: 10256

Solution structure of the C-terminal PH domain of hypothetical protein KIAA1914 from human

Authors

Li, H., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Hypothetical protein KIAA1914

Entity

1. Hypothetical protein KIAA1914 (polymer, Thiol state: all free), 107 monomers, 11770.93 Da Detail

GSSGSSGLET SSYLNVLVNS QWKSRWCSVR DNHLHFYQDR NRSKVAQQPL SLVGCEVVPD PSPDHLYSFR ILHKGEELAK LEAKSSEEMG HWLGLLLSES GSGPSSG

Formula weight

11770.93 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.3 %, Completeness: 94.2 %, Completeness (bb): 92.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.2 % (1140 of 1210)	94.9 % (598 of 630)	93.8 % (438 of 467)	92.0 % (104 of 113)
Backbone	92.1 % (582 of 632)	93.6 % (205 of 219)	91.3 % (284 of 311)	91.2 % (93 of 102)
Sidechain	96.1 % (649 of 675)	95.6 % (393 of 411)	96.8 % (245 of 253)	100.0 % (11 of 11)
Aromatic	98.0 % (98 of 100)	100.0 % (50 of 50)	95.7 % (45 of 47)	100.0 % (3 of 3)
Methyl	100.0 % (96 of 96)	100.0 % (48 of 48)	100.0 % (48 of 48)

1. PH domain

GSSGSSGLET SSYLNVLVNS QWKSRWCSVR DNHLHFYQDR NRSKVAQQPL SLVGCEVVPD PSPDHLYSFR ILHKGEELAK LEAKSSEEMG HWLGLLLSES GSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	PH domain	[U-13C; U-15N]	protein	1.59 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.27 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.27 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.03 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2COF, Strand ID: A Detail

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Release date

2009-11-19

Citation

Solution structure of the C-terminal PH domain of hypothetical protein KIAA1914 from human
Li, H., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Hypothetical protein KIAA1914, : 1 : 2 : 44 entities Detail

Interaction partners 1. Hypothetical protein KIAA1914, : 5 interactors Detail

Experiments performed 2 experiments Detail