BMRB: 10258

Solution structures of the fn3 domain of human ephrin type-B receptor 1

Authors

Sato, M., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Ephrin type-B receptor 1

Entity

1. Ephrin type-B receptor 1 (polymer, Thiol state: all free), 108 monomers, 11792.09 Da Detail

GSSGSSGPST VPIMHQVSAT MRSITLSWPQ PEQPNGIILD YEIRYYEKEH NEFNSSMARS QTNTARIDGL RPGMVYVVQV RARTVAGYGK FSGKMCFQTL TDSGPSSG

Formula weight

11792.09 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.3 %, Completeness: 96.5 %, Completeness (bb): 95.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.5 % (1166 of 1208)	96.5 % (607 of 629)	96.8 % (452 of 467)	95.5 % (107 of 112)
Backbone	95.4 % (605 of 634)	95.5 % (210 of 220)	95.8 % (300 of 313)	94.1 % (95 of 101)
Sidechain	96.1 % (645 of 671)	95.6 % (391 of 409)	96.8 % (243 of 251)	100.0 % (11 of 11)
Aromatic	97.8 % (88 of 90)	100.0 % (45 of 45)	95.5 % (42 of 44)	100.0 % (1 of 1)
Methyl	100.0 % (94 of 94)	100.0 % (47 of 47)	100.0 % (47 of 47)

1. Fibronectin type III domain

GSSGSSGPST VPIMHQVSAT MRSITLSWPQ PEQPNGIILD YEIRYYEKEH NEFNSSMARS QTNTARIDGL RPGMVYVVQV RARTVAGYGK FSGKMCFQTL TDSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	fn3 domain	[U-13C; U-15N]	protein	1 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.21 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.21 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.04 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.7 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DJS, Strand ID: A Detail

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Release date

2009-11-23

Citation

Solution structures of the fn3 domain of human ephrin type-B receptor 1
Sato, M., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Ephrin type-B receptor 1, : 1 : 196 entities Detail

Interaction partners 1. Ephrin type-B receptor 1, : 1 interactors Detail

Experiments performed 2 experiments Detail