BMRB: 10259

Solution structures of the PDZ domain of human unnamed protein product

Authors

Sato, M., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

human unnamed protein product

Entity

1. human unnamed protein product (polymer, Thiol state: all free), 104 monomers, 10523.67 Da Detail

GSSGSSGQAS GHFSVELVRG YAGFGLTLGG GRDVAGDTPL AVRGLLKDGP AQRCGRLEVG DLVLHINGES TQGLTHAQAV ERIRAGGPQL HLVIRRPLSG PSSG

Formula weight

10523.67 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.2 %, Completeness: 95.5 %, Completeness (bb): 95.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.5 % (1038 of 1087)	94.7 % (531 of 561)	97.1 % (409 of 421)	93.3 % (98 of 105)
Backbone	95.0 % (583 of 614)	93.8 % (210 of 224)	96.6 % (281 of 291)	92.9 % (92 of 99)
Sidechain	96.4 % (536 of 556)	95.3 % (321 of 337)	98.1 % (209 of 213)	100.0 % (6 of 6)
Aromatic	95.5 % (42 of 44)	95.5 % (21 of 22)	95.5 % (21 of 22)
Methyl	98.3 % (118 of 120)	98.3 % (59 of 60)	98.3 % (59 of 60)

1. PDZ domain

GSSGSSGQAS GHFSVELVRG YAGFGLTLGG GRDVAGDTPL AVRGLLKDGP AQRCGRLEVG DLVLHINGES TQGLTHAQAV ERIRAGGPQL HLVIRRPLSG PSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	PDZ domain	[U-13C; U-15N]	protein	1 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.0 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.0 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.09 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.0 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DJT, Strand ID: A Detail

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Release date

2009-11-23

Citation

Solution structures of the PDZ domain of human unnamed protein product
Sato, M., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. human unnamed protein product, : 1 : 351 entities Detail

Experiments performed 2 experiments Detail