BMRB: 10260

Solution structures of the fn3 domain of human receptor-type tyrosine-protein phosphatase F

Authors

Sato, M., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Receptor-type tyrosine-protein phosphatase F

Entity

1. Receptor-type tyrosine-protein phosphatase F (polymer, Thiol state: not present), 106 monomers, 10902.73 Da Detail

GSSGSSGPKP PIDLVVTETT ATSVTLTWDS GNSEPVTYYG IQYRAAGTEG PFQEVDGVAT TRYSIGGLSP FSEYAFRVLA VNSIGRGPPS EAVRARTGEQ SGPSSG

Formula weight

10902.73 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.5 %, Completeness: 93.4 %, Completeness (bb): 90.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.4 % (1037 of 1110)	93.3 % (530 of 568)	94.3 % (414 of 439)	90.3 % (93 of 103)
Backbone	90.9 % (562 of 618)	90.4 % (197 of 218)	91.7 % (278 of 303)	89.7 % (87 of 97)
Sidechain	96.2 % (561 of 583)	95.1 % (333 of 350)	97.8 % (222 of 227)	100.0 % (6 of 6)
Aromatic	100.0 % (82 of 82)	100.0 % (41 of 41)	100.0 % (40 of 40)	100.0 % (1 of 1)
Methyl	100.0 % (106 of 106)	100.0 % (53 of 53)	100.0 % (53 of 53)

1. Fibronectin type III domain

GSSGSSGPKP PIDLVVTETT ATSVTLTWDS GNSEPVTYYG IQYRAAGTEG PFQEVDGVAT TRYSIGGLSP FSEYAFRVLA VNSIGRGPPS EAVRARTGEQ SGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	fn3 domain	[U-13C; U-15N]	protein	1 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 2.55 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 2.55 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 2.93 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DJU, Strand ID: A Detail

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Release date

2009-11-23

Citation

Solution structures of the fn3 domain of human receptor-type tyrosine-protein phosphatase F
Sato, M., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Receptor-type tyrosine-protein phosphatase F, : 1 : 456 entities Detail

Interaction partners 1. Receptor-type tyrosine-protein phosphatase F, : 10 interactors Detail

Experiments performed 2 experiments Detail