BMRB: 10261

Solution structures of the WHEP-TRS domain of human methionyl-tRNA synthetase

Authors

Sato, M., Sasagawa, A., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Methionyl-tRNA synthetase

Entity

1. Methionyl-tRNA synthetase (polymer, Thiol state: not present), 79 monomers, 8183.260 Da Detail

GSSGSSGTTA KPQQIQALMD EVTKQGNIVR ELKAQKADKN EVAAEVAKLL DLKKQLAVAE GKPPEAPKGK KKKSGPSSG

Formula weight

8183.26 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 83.5 %, Completeness: 85.5 %, Completeness (bb): 83.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	85.5 % (761 of 890)	84.6 % (401 of 474)	86.8 % (290 of 334)	85.4 % (70 of 82)
Backbone	83.4 % (387 of 464)	82.0 % (132 of 161)	84.3 % (193 of 229)	83.8 % (62 of 74)
Sidechain	87.7 % (436 of 497)	85.9 % (269 of 313)	90.3 % (159 of 176)	100.0 % (8 of 8)
Methyl	100.0 % (78 of 78)	100.0 % (39 of 39)	100.0 % (39 of 39)

1. WHEP-TRS domain

GSSGSSGTTA KPQQIQALMD EVTKQGNIVR ELKAQKADKN EVAAEVAKLL DLKKQLAVAE GKPPEAPKGK KKKSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	WHWP-TRS domain	[U-13C; U-15N]	protein	1 mM
2	d-Tris HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.05 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.2 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DJV, Strand ID: A Detail

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Release date

2009-11-23

Citation

Solution structures of the WHEP-TRS domain of human methionyl-tRNA synthetase
Sato, M., Sasagawa, A., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Methionyl-tRNA synthetase, : 1 : 2 : 17 entities Detail

Interaction partners 1. Methionyl-tRNA synthetase, : 68 interactors Detail

Experiments performed 2 experiments Detail