BMRB: 10263

Solution structures of the 6th fn3 domain of human receptor-type tyrosine-protein phosphatase F

Authors

Sato, M., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Receptor-type tyrosine-protein phosphatase F

Entity

1. Receptor-type tyrosine-protein phosphatase F (polymer, Thiol state: all free), 107 monomers, 11666.92 Da Detail

GSSGSSGPGR PTMMISTTAM NTALLQWHPP KELPGELLGY RLQYCRADEA RPNTIDFGKD DQHFTVTGLH KGTTYIFRLA AKNRAGLGEE FEKEIRTPED LSGPSSG

Formula weight

11666.92 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.2 %, Completeness: 95.0 %, Completeness (bb): 94.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.0 % (1140 of 1200)	94.4 % (592 of 627)	95.7 % (447 of 467)	95.3 % (101 of 106)
Backbone	94.4 % (591 of 626)	92.7 % (203 of 219)	95.5 % (294 of 308)	94.9 % (94 of 99)
Sidechain	96.0 % (641 of 668)	95.3 % (389 of 408)	96.8 % (245 of 253)	100.0 % (7 of 7)
Aromatic	88.6 % (78 of 88)	88.6 % (39 of 44)	88.4 % (38 of 43)	100.0 % (1 of 1)
Methyl	100.0 % (94 of 94)	100.0 % (47 of 47)	100.0 % (47 of 47)

1. Fibronectin type III domain

GSSGSSGPGR PTMMISTTAM NTALLQWHPP KELPGELLGY RLQYCRADEA RPNTIDFGKD DQHFTVTGLH KGTTYIFRLA AKNRAGLGEE FEKEIRTPED LSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	fn3 domain	[U-13C; U-15N]	protein	1 mM
2	d-Tris HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.12 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.12 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.68 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DN7, Strand ID: A Detail

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Release date

2009-11-23

Citation

Solution structures of the 6th fn3 domain of human receptor-type tyrosine-protein phosphatase F
Sato, M., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Receptor-type tyrosine-protein phosphatase F, : 1 : 2 : 342 entities Detail

Interaction partners 1. Receptor-type tyrosine-protein phosphatase F, : 10 interactors Detail

Experiments performed 2 experiments Detail